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Terminal Transferase

from Calf Thymus, recombinant, E. coli

For general laboratory use.

Product No. Pack Size List Price Quantity
03333566001
8,000 U (for 20 tailing or 3'-end labeling reactions, 400 U per reaction) $196.00
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03333574001
24,000 U (for 60 tailing or 3'-end labeling reactions, 400 U per reaction) $478.00
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Terminal Transferase catalyzes the template independent addition of deoxy- and dideoxynucleoside triphosphates to the 3'-OH ends of double and single-stranded DNA fragments, and oligonucleotides. Terminal Transferase incorporates digoxigenin-, biotin-, and fluorochrome-labeled deoxy- and dideoxynucleoside triphosphates as well as radioactively labeled deoxy- and dideoxynucleoside triphosphates. The supplied 5x-concentrated reaction buffer allows the optimal tailing of all types of double-stranded DNA ends: blunt ended, with 3' overhang, or with 5' overhang. The highest incorporation rates are obtained with 3' overhangs.
 

Contents

  1. Terminal Transferase, 400 U/µl
  2. TdT Reaction Buffer, 5x concentrated
  3. CoCl2 Solution, 25 mM

 

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Roche Diagnostics Corporation
Roche Applied Science
9115 Hague Road
P.O. Box 50414
Indianapolis, IN 46250-0414 USA

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