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Gene Knockdown

Integrated Solutions for Gene Knockdown Analyses

Roche provides many integrated solutions for the application of RNAi in your research. Investigation of gene expression by RNA interference comprises a workflow of events which starts with the preparation of a suitable siRNA or the construction of an shRNA expression plasmid, usually followed by the transfection of these constructs into cultured cells. mRNA and protein analyses, as well as functional assays, are used to verify the effect of RNAi. Read on to discover the Roche reagents that can help drive your experimental RNAi workflow.

Achieve Consistent Transfection for Gene Knockdown
Short interfering RNA (siRNA) can be directly introduced into cells by transfection. The use of a lipid-based transfection reagent offers a convenient and reliable technique for the delivery of siRNAs into animal cells. X-tremeGENE siRNA Transfection Reagent is an optimized formulation that efficiently delivers siRNA into animal cells, resulting in both high protein expression and effective gene silencing. The reagent provides high transfection efficiency across a wide array of eukaryotic cell lines with minimal cytotoxicity. There is no need to change medium (e.g., to serum-free medium) prior to or following transfection.