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Gene Knockdown

Integrated Solutions for Gene Knockdown Analyses

Roche provides many integrated solutions for the application of RNAi in your research. Investigation of gene expression by RNA interference comprises a workflow of events which starts with the preparation of a suitable siRNA or the construction of an shRNA expression plasmid, usually followed by the transfection of these constructs into cultured cells. mRNA and protein analyses, as well as functional assays, are used to verify the effect of RNAi. Read on to discover the Roche reagents that can help drive your experimental RNAi workflow.

Fast and Efficient Plasmid Preparation

mRNA silencing by RNA interference is induced by double-stranded oligoribonucleotides with a length of about 22 base pairs with 2-nucleotide long 3' overhangs. This type of small dsRNA is called small interfering RNA or siRNA. For experimental purposes, siRNA can be produced by several methods in vitro and then transfected into the target cells, or it can be expressed in vivo using a specially designed expression plasmid. To achieve a high level of expression with a plasmid construct, a highly purified plasmid preparation must be used. The Genopure Kits provided by Roche allow you to prepare transfection-ready plasmids with a minimum of time and effort.