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[HomePageFeaturedProducts]
X-tremeGENE Transfection Reagents
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With 20 years of transfection experience, Roche offers two next-generation transfection reagents, X-tremeGENE HP and X-tremeGENE 9 Reagents, that deliver reliability and comprehensive efficiency across common and hard-to-transfect cell lines. Created to meet the needs of researchers who require high efficiency, ease-of-use, and reproducibility, X-tremeGENE Transfection Reagents provide more viable cells and more physiologically relevant results.
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X-tremeGENE HP DNA Transfection Reagent
X-tremeGENE HP DNA Transfection Reagent transfects a wide range of eukaryotic cells, including insect cells and difficult-to-transfect cells, such as primary cells. It is a unique non-liposomal reagent, free of animal-derived components, filtered through 0.2 µm pore size membrane, and active in serum. It complexes with nucleic acids, promoting efficient DNA transfer across cellular membranes, with minimal physiological and morphological cell changes. The supplied rapid protocol results in performance characteristics that make X-tremeGENE HP DNA Transfection Reagent the product of choice for cellular analysis and protein expression |
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X-tremeGENE 9 DNA Transfection Reagent
X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent well-suited for cellular analysis in a wide range of commonly used cell lines. X-tremeGENE 9 DNA Transfection Reagent shows very low cytotoxicity, is simple to use, and requires little optimization. This reagent produces high transfection efficiencies at low cell densities, even in the presence of serum. |
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X-tremeGENE siRNA Transfection Reagent
X-tremeGENE siRNA Transfection Reagent is an extraordinary multi-component reagent that forms a complex with siRNA, and enables effective delivery into eukaryotic cells. It shows high transfection efficiency in many commonly used cell types, including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several “hard-to-transfect” cell lines. In cotransfection-based RNAi experiments, X-tremeGENE siRNA Transfection Reagent enables both high protein expression and effective gene knockdown. This new reagent demonstrates low cytotoxicity, and functions exceptionally well independent of the presence or absence of serum, thereby eliminating the need to change media prior to transfection or after the addition of the transfection complex. |
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DOTAP Transfection Reagent
The monocationic chemical DOTAP is provided as a liposome formulation for the cationic liposome-mediated transfection of different negatively charged molecules into eukaryotic cells. Its highly effective performance has been demonstrated in a large number of publications, even for special applications, such as in vivo applications or the transfection of a variety of molecules (including DNA, RNA, oligonucleotides, nucleotides, proteins, and ribonucleoprotein complexes). |
Find more information on general transfection methods and success factors.
Related Products
Transfection with or without selection is only one part of a sequence of working steps that usually begins with the preparation of nucleic acids and ends with functional analysis. Roche Applied Science not only provides outstanding products for the transfection step, but also offers many reagents, kits, and other tools that can greatly facilitate your work.
Selection Antibiotics
Template Purification
Reporter Gene Assays
Gene Regulation Assays
Selection Antibiotics
Transient transfections are short-term transfections that utilize plasmid DNA as a temporary template for RNA transcription. Stable transfections occur when a chance recombination event takes place and foreign plasmid DNA is incorporated into the genome of the host cell. Cells that have undergone this event require specific selection in order to isolate them from the bulk of cells that did not incorporate the transfected DNA.
The most common method for the selection of stable transfectants involves antibiotics. To achieve this, the plasmid contains not only the gene of interest, but also an antibiotic-resistance gene. Alternatively, a second plasmid that contains an antibiotic-resistance gene is cotransfected. Roche Applied Science provides two antibiotics commonly used for the selection of stable transfectants.
Click here for selection antibiotics.
Template Purification
Preparation of nucleic acids is the first step in most molecular biology studies and all recombinant DNA techniques. Conventional methods for extraction and purification of nucleic acids are usually time-consuming, require the handling of toxic chemicals, and rely on expensive lab equipment, such as ultracentrifuges. To avoid these problems, Roche Applied Science provides a complete line of innovative products for the isolation and purification of RNA and DNA from practically any biological source.
Click here for purification products.
Reporter Gene Assays
Roche Applied Science offers a broad line of kits and reagent sets for reporter gene assays on transfected cells. These nonradioactive products measure promoter activity with sensitivity equal to or greater than that of radioisotopic assays, providing a safe alternative without sacrificing results.
The proteins that can be analyzed include chloramphenicol acetyltransferase (CAT), ß-galactosidase (ß-gal), luciferase (Luc), secreted alkaline phosphatase (SEAP), and human growth hormone (hGH).
Click here for reporter gene assays.
Gene Regulation Assays
Gene expression studies often require analysis of the steps involved in transcription. Roche Applied Science provides many kits for RNA and promoter analysis.
Click here for gene regulation assays.
General Recommendation for Transfection Reagent Selection
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X-tremeGENE HP DNA Transfection Reagent |
X-tremeGENE 9 DNA Transfection Reagent |
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| Transfection of DNA | +++ | +++ |
| Efficiency with standard cell lines (e.g., COS-7, HEK-293, HeLa, NIH 3T3, and CHO-K1) | +++ | +++ |
| Efficiency with "difficult-to-transfect" cells (e.g., HT 29, HCT 116, and K-562 cells) | +++ | +(+) |
| Transfection of primary cells | ++(+) | NR |
| Gentleness of the reagent | ++(+) | +++ |
| Ease of use (minimal optimization) | +++ | +++ |
| Cytotoxicity after transfection | Very low | Very low to exceptionally low |
NR: Not Recommended
Focus Application |
X-tremeGENE HP DNA Transfection Reagent |
X-tremeGENE 9 DNA Transfection Reagent |
X-tremeGENE siRNA Transfection Reagent |
| Functional studies of cellular processes | +++ | +++ | +++ |
| Quantitative protein expression | +++ | ++ | - |
| Gene knockdown studies using siRNA | - | - | +++ |
Cell-Type Specific Product Recommendations
Established Cell Lines |
X-tremeGENE HP DNA Transfection Reagent |
X-tremeGENE 9 DNA Transfection Reagent |
| CHO-K1 | ++(+) | +++ |
| COS-7 | ++(+) | ++(+) |
| NIH-3T3 | +(+) | ++(+) |
| HeLa | ++(+) | +++ |
| HEK-293 | +++ | ++ |
| Hep-G2 | +(+) | ++ |
| HT1080 | ++ | +++ |
| HT29 | + | NR |
| PC-3 | ++ | ++ |
| K-562 | +++ | NR |
| U-2 OS | + | NR |
| MCF-7 | + | +++ |
| RAW | + | NR |
| HCT 116 | +++ | ++ |
| Jurkat | + | NR |
| PC-12 | ++ | ++ |
| Primary Cells | ||
| Human primary fibroblasts | +(+) | NR |
| MEF | ++ | NR |
| Stem Cells | ||
| Human mesenchymal stem cells | +(+) | NR |
| Mouse embryonic stem cells (E14TG2a) | +(+) | ++ |
| Insect Cell Lines | ||
| High Five | ++ | NR |
| SF9 | ++ | NR |
NR: Not Recommended
Note: The above data was produced using either a GFP-encoding pcDNA3.1 plasmid or a luciferase-encoding pCI plasmid, both with the cytomegalovirus (CMV) promoter. These recommendations are guidelines based on experimental findings. The optimal reagent:DNA ratio must be determined empirically.
Application Table
Focus Applications |
Recommended Product |
Advantages |
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Search Roche's comprehensive database of peer-reviewed publications and positive customer feedback.
Cell-type specific protocols and results
Experiments below show high level gene expression in mammalian and insect cells transfected using X-tremeGENE Transfection Reagents.
Fast straightforward transfection protocols can be obtained by clicking the cell type below. These protocols are guidelines. Optimal transfection conditions must be determined empirically for your cell culture conditions.
- X-tremeGENE HP DNA Transfection Reagent protocols
- X-tremeGENE 9 DNA Transfection Reagent protocols
- X-tremeGENE siRNA Transfection Reagent protocols
Protocols and results provided by customers
Protocols shown here are provided by customers and have not been verified by Roche scientists. These protocols are guidelines. Optimal transfection conditions must be determined empirically for your cell culture conditions.
- X-tremeGENE HP DNA Transfection Reagent customer protocols
- X-tremeGENE 9 DNA Transfection Reagent customer protocols
X-tremeGENE HP DNA Transfection Reagent
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Cell name |
Cell type |
Transfected reporter gene |
Customer protocol(s) |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
GFP |
- |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
GFP / EGFP-CB2 / Not indicated |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
Stable transfection |
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Human embryonic kidney cell line |
GFP |
- |
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Human embryonic kidney cell line subclone |
Luciferase |
- |
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293FT |
Human embryonic kidney cell line subclone |
GFP |
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293T |
Human embryonic kidney cell line expressing SV40 large T antigen |
GFP / Interleukin / Fc fusion protein |
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3T3 (ATCC®CRL-1658™) |
Mouse embryonic fibroblasts |
GFP |
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3T3-L1 (ATCC®CRL-173™) |
Mouse embryonic fibroblasts |
SEAP |
- |
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A549 (ATCC®CCL-185™) |
Human carcinomic alveolar basal epithelial cells |
SEAP and EGFP |
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AGS (ATCC®CRL-1739™) |
Human epithelial adenocarcinoma cells |
GFP |
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C2C12 (ATCC® CRL-1772™) |
Mouse myoblast cell line |
SEAP and EGFP |
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CHO/dhFr- (ATCC® CRL-9096™) |
Chinese hamster ovary cell line subclone |
Secretable protein |
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CHO-K1 (ATCC® CCL-61™) |
Chinese hamster ovary cell line subclone |
GFP |
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Chinese hamster ovary cell line subclone |
Luciferase |
- |
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COS-7 (ATCC® CRL-1651™) |
African green monkey kidney fibroblast-like cell line |
GFP |
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Dental papilla mesenchymal cells |
Primary cells from the first lower molar of E18.5 Swiss mice |
GFP |
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EC109 |
Esophageal squamous carcinoma cell line |
Not indicated |
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EC9706 |
Esophageal squamous carcinoma cell line |
Not indicated |
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F9 (ATCC® CRL-1720™) |
Mouse embryonal carcinoma cells |
EGFP / GFP |
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Human primary fibroblasts |
GFP |
- |
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Human embryonic stem cells |
GFP |
- |
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HCT 116 (ATCC® CCL-™) |
Human colorectal carcinoma cell line |
Luciferase |
- |
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HeLa (ATCC® CCL-2™) |
Human cervix carcinoma cell line |
GFP |
- |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP |
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HepG2.2.15 |
Human hepatoma cell line expressing complete HBV genome |
GFP |
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Insect cell line (Invitrogen) |
ß-gal |
- |
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HT-29 (ATCC® HTB-38™) |
Human colorectal adenocarcinoma cell line |
GFP |
- |
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HT-1080 (ATCC® CCL 121™) |
Human fibrosarcoma cell line |
GFP |
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HuH-7 (ATCC® JCRB-0403) |
Human hepatoma cell line |
GFP |
- |
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HuH-7.5 |
Human hepatoma cell line |
EGFP |
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Human lymphoblast cell line |
GFP |
- |
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K-562 (ATCC® CCL 243™) |
Human immortalised myelogenous leukemia cell line |
GFP |
- |
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L02 |
Immortal hepatic cell line |
RNAi plasmid encoding GFP |
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LNCaP (ATCC® CRL-1740™) |
Human prostate cancer cell line |
GFP |
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MC3T3-E1 (ATCC® CRL-2593™) |
Mouse osteoblast-like cells |
sheep C12orf29 protein |
- |
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MCF7 (ATCC® HTB-22™) |
Human adenocarcinoma cell line |
GFP |
- |
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MDA-MB-231 (ATCC® HTB-26™) |
Human adenocarcinoma cell line |
SEAP |
- |
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Primary mouse embryonic fibroblasts |
GFP |
- |
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Human lung fibroblast cells |
GFP |
- |
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PC-3 (ATCC® CRL-1435™) |
Human prostate adenocarcinoma cell line |
GFP |
- |
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PC-12 (ATCC® CRL-1721™) |
Rat adrenal pheochromocytoma cell line |
Luciferase |
- |
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Plat-E |
Human kidney packaging cell line |
GFP |
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Porcine ovarian granulosa cells |
Primary cells |
EGFP |
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PK-15 (ATCC®CCL-33™) |
Porcine kidney epithelial cell line |
GFP |
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RAW 264.7 (ATCC® TIB-71™) |
Mouse monocyte macrophage cell line |
GFP |
- |
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Epithelial Drosophila melanogaster cell line |
GFP |
- |
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Sf9 (ATCC® TIB-71™) |
Epithelial fall armyworm (Spodoptera frugiperda) cells |
ß-gal |
- |
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Human mesenchymal stem cells |
GFP |
- |
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Stems cells, mesenchymal |
Murine mesenchymal stem cells (RM26/2) |
Far-red fluorescent protein |
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Stems cells, mesenchymal |
Murine mesenchymal stem cells (EK8) |
Far-red fluorescent protein |
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Stems cells, mesenchymal |
Murine mesenchymal stem cells (p53-/-) |
Far-red fluorescent protein |
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U-2 OS (ATCC® HTB-96™) |
Human osteosarcoma cell line |
GFP |
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U-87 MG (ATCC® HTB-14™) |
Human epithelial glioblastoma cells |
GFP |
For further information on cells transfected with X-tremeGENE HP DNA Transfection Reagent you can also browse the Transfection Database.
X-tremeGENE 9 DNA Transfection Reagent
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Cell name |
Cell type |
Transfected reporter gene |
Customer protocol(s) |
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293-A |
Human embryonic kidney cell line |
EGFP |
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293FT |
Human embryonic kidney cell line subclone |
Luciferase |
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293T |
Human embryonic kidney cell line |
Luciferase / EGFP / Flag-tagged protein |
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BF-2 (ATCC® CCL-91™) |
Human embryonic kidney cell line |
RFP and EGFP |
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C2C12 (ATCC® CRL-1772™) |
Mouse myoblast cell line |
EGFP |
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CHO-K1 (ATCC® CCL-61™) |
Chinese hamster ovary cell line subclone |
GFP |
- |
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CHSE-214 (ATCC® CRL-1681™) |
Chinook salmon embryo (fish cell line) |
EGFP |
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COS-7 (ATCC® CRL-1651™) |
African green monkey kidney fibroblast-like cell line |
GFP |
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EPC (ATCC® CRL-2872™) |
Fish cell line (Epithelioma papulosum cyprini) |
RFP |
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ES-E14TG2a (ATCC® CRL-8121™) |
Mouse embyonic stem cells |
GFP |
- |
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GF |
Fish cell line (grouper fin) |
RFP and EGFP |
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HCT 116 (ATCC® CCL-™) |
Human colorectal carcinoma cell line |
Luciferase |
- |
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HeLa (ATCC® CCL-2™) |
Human cervix carcinoma cell line |
GFP, EGFP-actin |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP |
- |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
14 kb plasmid (incl. eGFP; stable) |
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HINAE |
Japanese flounder (Hirame Natural Embryo) fish cell line |
RFP and EGFP |
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HT-1080 (ATCC® CCL-121™) |
Human fibrosarcoma cell line |
GFP |
- |
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HuH-7 (ATCC® JCRB-0403) |
Human hepatoma cell line |
Luciferase |
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MCF7 (ATCC® HTB-22™) |
Human adenocarcinoma cell line |
GFP |
- |
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Neuro-2a (ATCC® CCL-131™) |
Mouse neuroblastoma cells |
GFP-mortalin fusion protein |
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NIH-3T3 (ATCC® CRL-1658™) |
Mouse embryonic cell line (fibroblast) |
GFP |
- |
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PC-3 (ATCC® CRL-1435™) |
Human prostate adenocarcinoma cell line |
GFP |
- |
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PC-12 (ATCC® CRL-1721™) |
Rat adrenal pheochromocytoma cell line |
Luciferase |
- |
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Primary pulmonary fibroblasts |
Human pulmonary fibroblasts (primary) |
Not indicated |
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Porcine granulosa cells |
Porcine granulosa cells (subculture of primary cells) |
GFP fusion protein |
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Sf9 (ATCC® TIB-71™) |
Epithelial fall armyworm (Spodoptera frugiperda) cells |
GFP |
For further information on cells transfected with X-tremeGENE 9 DNA Transfection Reagent you can also browse the Transfection Database.
X-tremeGENE siRNA Transfection Reagent
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Cell Type |
X-tremeGENE |
siRNA1 |
Culture Dish |
Total Volume |
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CHO-K1 (ATCC® CCL-61™) |
10 µl |
2 µg |
24-well plate |
550 µl |
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COS-7 (ATCC® CRL-1651™) |
2.5 µl |
0.5 µg |
24-well plate |
550 µl |
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HEK-293 (ATCC® CRL-1573™) |
5 µl |
0.5 µg |
24-well plate |
550 µl |
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HeLa (ATCC® CCL-2™) |
5 µl |
0.5 µg |
24-well plate |
550 µl |
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Hep G2 (ATCC® HB-8065™) |
10 µl |
2 µg |
24-well plate |
550 µl |
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HT-29 (ATCC® HTB-38™) |
10 µl |
2 µg |
24-well plate |
550 µl |
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HTC-116 (ATCC® CCL-247™) |
2.5 µl |
0.5 µg |
24-well plate |
550 µl |
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Huh-7 clone 9B |
10 µl |
1 µg |
6-well plate |
1000 µl2 |
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MCF7 (ATCC® HTB-22™) |
2.5 µl |
0.5 µg |
24-well plate |
550 µl |
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NIH 3T3 (ATCC® CRL-1658™) |
5 µl |
0.5 µg |
24-well plate |
550 µl |
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PC-3 (ATCC® CRL-1435™) |
5 µl |
0.5 µg |
24-well plate |
550 µl |
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PC-12 (ATCC® CRL-1721™) |
2.5 µl |
0.5 µg |
24-well plate |
550 µl |
10.5 µg corresponds to 67.5 nM siRNA
2medium changed 5 hours after transfection
For further information on cells transfected with X-tremeGENE siRNA Transfection Reagent you can also browse the Transfection Database.
Protocols and results provided by customers
Protocols shown here are provided by customers and can be obtained by clicking the cell type below. These protocols have not been verified by Roche scientists. These protocols are guidelines. Optimal transfection conditions must be determined empirically for your cell culture conditions.
Note: Data and experimental conditions included in "Customer transfection protocols" are the sole responsibility of the customers that have submitted them. Roche was neither involved in establishing the experimental conditions nor in defining the criteria for the performance of the specific assays. Roche therefore cannot take any responsibility for performance or interpretation of results obtained for the biological target parameter(s) described by the authors or other users using a similar experimental approach.
X-tremeGENE HP DNA Transfection Reagent
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Cell name |
Cell type |
Transfected reporter gene |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
Not indicated |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
GFP |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
EGFP-CB2 |
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293 (ATCC® CRL-1573™) |
Human embryonic kidney cell line |
Stable transfection |
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Human embryonic kidney cell line |
EGFP fusion protein |
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Human embryonic kidney cell line subclone |
GFP |
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Human embryonic kidney cell line subclone |
Luciferase |
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Human embryonic kidney cell line subclone |
Pelota/Ubc9 |
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Human embryonic kidney cell line expressing SV40 large T antigen |
GFP |
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Human embryonic kidney cell line expressing SV40 large T antigen |
Interleukin /Fc fusion protein |
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3T3 (ATCC®CRL-1658™) |
Mouse embryonic fibroblasts |
GFP |
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A549 (ATCC®CCL-185™) |
Human carcinomic alveolar basal epithelial cells |
EGFP |
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AGS (ATCC®CRL-1739™) |
Human epithelial adenocarcinoma cells |
GFP |
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C2C12 (ATCC®CRL-1772™) |
Mouse myoblast cell line |
EGFP |
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CHO/dhFr- (ATCC® CRL-9096™) |
Chinese hamster ovary cell line subclone |
Secretable protein |
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CHO-K1 (ATCC® CCL-61™) |
Chinese hamster ovary cell line subclone |
GFP |
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COS-7 (ATCC® CRL-1651™) |
African green monkey kidney fibroblast-like cell line |
GFP |
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Primary cells from the first lower molar of E18.5 Swiss mice |
GFP |
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Esophageal squamous carcinoma cell line |
Not indicated |
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Esophageal squamous carcinoma cell line |
Not indicated |
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F9 (ATCC® CRL-1720™) |
Mouse embryonal carcinoma cells |
EGFP |
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F9 (ATCC® CRL-1720™) |
Mouse embryonal carcinoma cells |
GFP |
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F9 (ATCC® CRL-1720™) |
Mouse embryonal carcinoma cells |
GFP |
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HCT 116 (ATCC® CCL-™) |
Human colorectal carcinoma cell line |
Not indicated |
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HeLa (ATCC® CCL-2™) |
Human cervix carcinoma cell line |
EGFP fusion protein |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP |
|
HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP |
|
HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
GFP and shRNA |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
HBV genome |
|
Human hepatoma cell line expressing complete HBV genome |
GFP |
|
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HT-1080 (stably transfected) |
Human fibrosarcoma cell line |
EGFP-120 kDa nuclear protein |
|
HuH-7 (ATCC® JCRB-0403) |
Human hepatoma cell line |
Hepatitis B surface antigen |
|
HuH-7 (ATCC® JCRB-0403) |
Human hepatoma cell line |
Luciferase |
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Human hepatoma cell line |
EGFP |
|
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Immortal hepatic cell line |
RNAi plasmid encoding GFP |
|
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LNCaP (ATCC® CRL-1740™) |
Human prostate cancer cell line |
GFP |
|
MCF7 (ATCC® HTB-22™) |
Human adenocarcinoma cell line |
HA-tagged protein |
|
MCF7 (ATCC® HTB-22™) |
Human adenocarcinoma cell line |
Not indicated |
|
MEF (ATCC® HTB-22™) |
Primary mouse embryonic fibroblasts |
Not indicated |
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Human kidney packaging cell line |
GFP |
|
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PK-15 (ATCC®CCL-33™) |
Porcine kidney epithelial cell line |
GFP |
|
Primary cells |
EGFP |
|
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Murine mesenchymal stem cells (RM26/2) |
Far-red fluorescent protein |
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Murine mesenchymal stem cells (EK8) |
Far-red fluorescent protein |
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Murine mesenchymal stem cells (p53-/-) |
Far-red fluorescent protein |
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U-2 OS (ATCC® HTB-96™) |
Human osteosarcoma cell line |
GFP |
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U-87 MG (ATCC® HTB-14™) |
Human epithelial glioblastoma cells |
GFP |
For further information on cells transfected with X-tremeGENE HP DNA Transfection Reagent you can also browse the Transfection Database.
X-tremeGENE 9 DNA Transfection Reagent
|
Cell name |
Cell type |
Transfected reporter gene |
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Human embryonic kidney cell line |
EGFP |
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Human embryonic kidney cell line subclone |
Luciferase |
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Human embryonic kidney cell line |
Luciferase |
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Human embryonic kidney cell line |
EGFP |
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Human embryonic kidney cell line |
Flag-tagged protein |
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BF-2 (ATCC® CCL-91™) |
Bluegill fry (fish cell line) |
RFP and EGFP |
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C2C12 (ATCC® CRL-1772™) |
Mouse myoblast cell line |
EGFP |
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CHSE-214 (ATCC® CRL-1681™) |
Chinook salmon embryo (fish cell line) |
EGFP |
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COS-7 (ATCC® CRL-1651™) |
African green monkey kidney fibroblast-like cell line |
EGFP-fusion protein |
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COS-7 (ATCC® CRL-1651™) |
African green monkey kidney fibroblast-like cell line |
GFP |
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EPC (ATCC® CRL-2872™) |
Fish cell line (Epithelioma papulosum cyprini) |
RFP |
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Fish cell line (grouper fin) |
RFP and EGFP |
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HeLa (ATCC® CCL-2™) |
Human cervix carcinoma cell line |
EGFP-actin |
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HeLa (ATCC® CCL-2™) |
Human cervix carcinoma cell line |
GFP |
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HepG2 (ATCC® HB-8065™) |
Human hepatoma cell line |
14 kb plasmid (incl. eGFP; stable) |
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Japanese flounder (Hirame Natural Embryo) fish cell line |
RFP and EGFP |
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HuH-7 (ATCC® JCRB-0403) |
Human hepatoma cell line |
Luciferase |
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MCF7 (ATCC® HTB-22™) |
Human adenocarcinoma cell line |
FAK-enconding shRNA plasmid |
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Neuro-2a (ATCC® CCL-131™) |
Mouse neuroblastoma cells |
GFP-mortalin fusion protein |
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PC-12 (ATCC® CRL-1721™) |
Rat adrenal pheochromocytoma cell line |
EGFP fusion protein |
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Human pulmonary fibroblasts (primary) |
Not indicated |
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Porcine granulosa cells (subculture of primary cells) |
GFP fusion protein |
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Sf9 (ATCC® TIB-71™) |
Epithelial fall armyworm (Spodoptera frugiperda) cells |
GFP |
For further information on cells transfected with X-tremeGENE 9 DNA Transfection Reagent you can also browse the Transfection Database.
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cOmplete ULTRA Tablets
The Next Step in Protein Protection
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