Related products
Glycogen
Glycogen is used as a carrier for the precipitation of nucleic acids (DNA or
RNA). As an inert material, it may replace tRNA or sonicated DNA. 20 µg
glycogen (1 µl solution) allows the precipitatation of picogram (pg) amounts
of DNA or RNA from a volume of 1 ml.
Human Genomic
DNA
Human Genomic DNA contains high molecular weight genomic DNA isolated from human
blood (buffy coat) using the method of Sambrook et al. The DNA is supplied as
a solution in 10 mM Tris/HCl, 1 mM EDTA, pH 8.0.
Human t-PA Control
Primer Set
Human tPA Control Primer Set contains five different primers allowing amplification
of 4.8 kb, 9.3 kb and 15 kb fragments of the t-PA gene from human genomic DNA.
MgCl2
Stock Solution
The MgCl2 Stock Solution is provided at a concentration of 25 mM
MgCl2 in sterile water. It is optimized for use in PCR.
Oligo(dT)20
Probe, Biotin labeled
Use biotin-labeled oligo (dT)20 probe for hybridization with eucaryotic mRNA
and immobilisation on streptavidin-coated solid supports like magnetic particles
or PCR tubes.
PCR Buffer Set
Optimal concentration of MgCl2 increases the specificity and yield
of PCR. The PCR Buffer Set contains MgCl2 solution, and a PCR buffer
without MgCl2.The buffer in combination with the MgCl2
stock solution allows individual adjustment of the Mg2+ concentration
in the PCR. In most applications a concentration of 1.5 mM MgCl2
yields satisfactory results with a dNTP concentration of 200 µM each.
PCR Buffer without
MgCl2 10x conc.
The PCR Buffer without MgCl2 is a solution containing 100 mM Tris-HCl,
500 mM KCl at pH 8.3 (20°C). The buffer is optimized for the amplification
of specific DNA-fragments by the polymerase chain reaction (PCR). The buffer
is ten-fold concentrated and should be used in combination with the MgCl2
Stock Solution.
Protector RNase
Inhibitor
Protector RNase Inhibitor is used to protect mRNA. Protector RNase Inhibitor
inhibits a wide spectrum of different RNases. It is useful in any application
where RNases could be a potential problem.
Streptavidin-coated PCR Tubes (Strips)
Strip PCR Tubes
and Caps
8- and 12-Strip PCR Tubes and Caps are designed
for use in PCR thermal cyclers, and provide optimal and rapid heat transfer.
T4 Gene 32 Protein
T4 gene 32 protein is a single-strand specific and thus a helix-destabilizing
protein encoded by gene 32 of the phage T4 genome. The protein is an essential
component of the T4 DNA replication system and plays an important role in both
DNA replication and recombination in T4-infected cells.
Tth Pyrophosphatase,
thermostable
Tth pyrophosphatase (PPase), originally isolated from the thermophilic
eubacterium Thermus thermophilus, eliminates inorganic pyrophosphate
(PPi) created by incorporation of nucleotide triphosphates (dNTPs) during PCR.
Pyrophosphate is reported to inhibit DNA polymerase activity and causes DNA
and RNA degradation at elevated temperatures. By eliminating PPi, PPase helps
prevent inhibition and degradation problems.
Uracil-DNA Glycosylase
Uracil-DNA Glycosylase (UNG) hydrolyzes uracil-glycosidic bonds in single- or
double-stranded DNA excising uracil and creating alkali-sensitive abasic sites
in the DNA. These abasic sites can be hydrolyzed by endonuclease, heat or alkali
treatment (U-DNA cleavage). U-DNA cleavage can be used in all applications when
the removal of template DNA is needed for higher efficiency, e.g.,
to increase the efficiency of site-directed mutagenesis, or the prevention of
carry-over contamination.
Uracil-DNA Glycosylase,
heat-labile
Use Uracil-DNA Glycosylase (UNG) to hydrolyze uracil-glycosidic bonds in single-
or double-stranded DNA excising uracil and creating alkali-sensitive abasic
sites in DNA. These abasic sites can be hydrolyzed by endonuclease, heat or
alkali treatment (U-DNA cleavage). In addition to experiments involving site-directed
mutagenesis, the heat-labile Uracil-DNA Glycosylase (UNG) is ideally suited
for preventing carryover contamination between PCRs.
Water, PCR Grade
Water, PCR Grade is specially purified, double-distilled, deionized, autoclaved
and free of detectable endonucleases, ribonucleases, and nicking activity. It
is routinely function tested in PCR. The unique production process also ensures
that no detectable amounts of nucleic acids are included, as tested with conventional
thermal cyclers and in highly sensitive assays with the LightCycler Instrument
and related kits.
Isolation and Purification of Nucleic Acids
Proteinase K,
recombinant, PCR Grade (Lyophilizate)
Proteinase K is one of the most active endopeptidases known and does not exhibit
any pronounced cleavage specificity. The Proteinase K, PCR Grade is particularly
well suited for isolating nucleic acids for amplification reactions. This preparation
is tested for the absence of RNases and DNases, and is also free from DNA. The
enzyme is supplied as a lyophilizate or as a solution.
Proteinase K,
recombinant, PCR Grade (Solution)
Proteinase K is one of the most active endopeptidases known and does not exhibit
any pronounced cleavage specificity. The Proteinase K, PCR Grade is particularly
well suited for isolating nucleic acids for amplification reactions. This preparation
is tested for the absence of RNases and DNases, and is also free from DNA. The
enzyme is supplied as a lyophilizate or as a solution.
Labeling and Detection of Nucleic Acids
DNA Polymerase
I, Endonuclease-free
DNA Polymerase I is the recombinant form of the E. coli DNA polymerase
- the product of the E. coli pol A gene. This gene has been cloned
into an overproducing lysogenic strain. Each enzyme preparation is checked for
the absence of endonucleases.
DNA Polymerase
I, Nick Translation Grade
DNA Polymerase I is the recombinant form of the E. coli DNA polymerase
- the product of the E. coli pol A gene. This gene has been cloned
into an overproducing lysogenic strain. This special formulation for use in
nick translation labeling of DNA, does not require addition of DNase I.
Klenow Enzyme,
Labeling Grade
Klenow enzyme, Labeling grade is used for the second-strand synthesis
of cDNA, sequencing DNA according to Sanger by the dideoxy-chain termination
method and for elongation of oligonucleotides in site-directed mutagenesis.
Klenow Enzyme catalyzes the addition of mononucleotides from deoxynucleoside-5’-triphosphates
to the 3’-hydroxyl terminus of a primer/template DNA.
Klenow Enzyme,Sequencing
Grade
Klenow enzyme, Sequencing Grade, is used for the second-strand synthesis of
cDNA, sequencing DNA according to Sanger by the dideoxy-chain termination method
and for elongation of oligonucleotides in site-directed mutagenesis. Sequencing
Grade Klenow Enzyme is tested for the absence of unspecific endonucleases and
exonucleases, and the performance in sequencing experiments.
SP6 RNA Polymerase
SP6 RNA Polymerase is used to transcribe RNA from DNA templates, cloned into
vectors with the appropriate phage promoter. Homogeneously labeled RNA can be
synthesized with high efficiency and used as hybridization probes in Southern,
Northern, and dot blots, as well as in-situ hybridizations. Transcripts can
be nonradioactively labeled with Biotin-16-UTP or DIG-11-UTP or Fluorescein-12-UTP,
or radioactively labeled to high specific activity with [alpha-32P] or [alpha-35S]
labeled nucleotides. Also available are nucleotide mixes (10x conc.) for DIG,
biotin, and fluorescein RNA labeling that give optimal results with SP6 RNA
Polymerase and the supplied 10x reaction buffer.
T3 RNA Polymerase
T3 RNA Polymerase is used to transcribe RNA from DNA templates, cloned into
vectors with the appropriate phage promoter. Homogeneously labeled RNA can be
synthesized with high efficiency and used as hybridization probes in Southern,
Northern, and dot blots, as well as in-situ hybridizations. Transcripts can
be nonradioactively labeled with Biotin-16-UTP or DIG-11-UTP or Fluorescein-12-UTP,
or radioactively labeled to high specific activity with [alpha-32P] or [alpha-35S]
labeled nucleotides. Also available are nucleotide mixes (10x conc.) for DIG,
biotin, and fluorescein RNA labeling that give optimal results with T3 RNA Polymerase
and the supplied 10x reaction buffer.
T4 DNA Polymerase
T4 DNA Polymerase is a DNA dependent DNA polymerase that catalyzes the polymerization
of deoxynucleoside-5´-triphosphates to the hydroxyl termini of recessive
ends. Blunt-ended DNA cannot serve as a template for the polymerization reaction.
T7 RNA Polymerase
T7 RNA Polymerase is commonly used to transcribe DNA which has been cloned into
vectors which have two phage promoters in opposite orientation. RNA can be selectively
synthesized from either strand of the insert DNA with different polymerases.
Homogeneously labeled single-stranded RNA can be generated with this system.
Transcripts can be nonradioactively labeled with Biotin-16-UTP or DIG-11-UTP
or radioactively labeled to high specific activity with [alpha-32P] or [alpha-35S]
labeled nucleotides.
COT Human DNA
Use COT Human DNA for suppressing crosshybridization to human repetitive DNA
in filter and microarray hybridizations and in situ hybridization experiments.
COT Human DNA is prepared from human placental DNA by shearing, denaturing,
and reannealing under conditions that enrich these repetitive elements.
Anti-Digoxigenin-POD, Fab Fragments
Mapping and Cloning of Nucleic Acids
DNA, lambda (lambda
DNA)
Use this high quality preparation of Lambda DNA as a template in amplification
reactions, or control element (e.g., PCR and restriction digests).
It is isolated from bacteriophage lambda (cI857 Sam 7). Lambda DNA has a molecular
weight of 32 300 kD and is 48,502 base pairs in length.
DNA Molecular
Weight Marker II
Rely on DNA Molecular Weight Marker II for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.12 - 23.1 kbp showing 8 fragments of:
125, 564, 2027, 2322, 4361, 6557, 9416, 23130 bp.
DNA Molecular
Weight Marker II, DIG-labeled
Rely on DNA Molecular Weight Marker II for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.12 - 23.1 kbp showing 8 fragments of:
125, 564, 2027, 2322, 4361, 6557, 9416, 23130 bp. The DIG-labeled version can
be used as size standards in Southern blot analysis when the DIG System for
Nucleic Acid Labeling and Detection is used.
DNA Molecular
Weight Marker III
Rely on DNA Molecular Weight Marker III for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.12 - 21.2 kbp showing 13 fragments
of: 125, 564, 831,947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, 21226
bp.
DNA Molecular
Weight Marker III, DIG-labeled
Rely on DNA Molecular Weight Marker III for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.12 - 21.2 kbp showing 13 fragments
of: 125, 564, 831,947, 1375, 1584, 1904, 2027, 3530, 4268, 4973, 5148, 21226
bp. The DIG-labeled version can be used as size standards in Southern blot analysis
when the DIG System for Nucleic Acid Labeling and Detection is used.
DNA Molecular
Weight Marker IV
Rely on DNA Molecular Weight Marker IV for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.07 - 19.3 kbp showing 14 fragments
of: 74, 421, 697, 925, 1150, 1489, 1882, 2322, 2690, 3140, 4254, 5526, 7743,
19329 bp.
DNA Molecular
Weight Marker V
Rely on DNA Molecular Weight Marker V for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 8 – 587 bp showing 22 fragments
of: 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267,
434, 458, 504, 540, 587 bp.
DNA Molecular
Weight Marker V, DIG-labeled
Rely on DNA Molecular Weight Marker V for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 8 – 587 bp showing 22 fragments
of: 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267,
434, 458, 504, 540, 587 bp. The DIG-labeled version can be used as size standards
in Southern blot analysis when the DIG System for Nucleic Acid Labeling and
Detection is used.
DNA Molecular
Weight Marker VI
Rely on DNA Molecular Weight Marker VI for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.15 - 2.1 kbp showing 15 fragments of:
154, 154, 220, 234, 234, 298, 298, 394, 453, 517, 653, 1033, 1230, 1766, 2176
bp.
DNA Molecular
Weight Marker VI, DIG-labeled
Rely on DNA Molecular Weight Marker VI for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.15 - 2.1 kbp showing 15 fragments of:
154, 154, 220, 234, 234, 298, 298, 394, 453, 517, 653, 1033, 1230, 1766, 2176
bp. The DIG-labeled version can be used as size standards in Southern blot analysis
when the DIG System for Nucleic Acid Labeling and Detection is used.
DNA Molecular
Weight Marker VII
Rely on DNA Molecular Weight Marker VII for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.081 - 8.57 kbp showing 17 fragments
of: 81, 359, 492, 710, 718, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899,
6106, 7427, 8576 bp.
DNA Molecular
Weight Marker VII, DIG-labeled
Rely on DNA Molecular Weight Marker VII for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.081 - 8.57 kbp showing 17 fragments
of: 81, 359, 492, 710, 718, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899,
6106, 7427, 8576 bp. The DIG-labeled version can be used as size standards in
Southern blot analysis when the DIG System for Nucleic Acid Labeling and Detection
is used.
DNA Molecular
weight Marker VIII
Rely on DNA Molecular Weight Marker VIII for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 19 - 1114 bp showing 18 fragments of:
19, 26, 34, 34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900,
1114 bp.
DNA Molecular
weight Marker VIII, DIG-labeled
Rely on DNA Molecular Weight Marker VIII for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 19 - 1114 bp showing 18 fragments of:
19, 26, 34, 34, 37, 67, 110, 124, 147, 190, 242, 320, 404, 489, 501, 692, 900,
1114 bp. The DIG-labeled version can be used as size standards in Southern blot
analysis when the DIG System for Nucleic Acid Labeling and Detection is used.
DNA Molecular
Weight Marker IX
Rely on DNA Molecular Weight Marker IX for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 72 – 1353 bp showing 11 fragments
of: 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, 1353 bp.
DNA Molecular
Weight Marker X
Rely on DNA Molecular Weight Marker X for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 0.07 – 12.2 kbp showing 23 fragments
of: 75, 134, 154, 201, 220, 298, 344, 396, 506, 517, 1018, 1636, 2036, 3054,
4072, 5090, 6108, 7126, 8144, 9162, 10180, 11198, 12216 bp.
DNA Molecular
Weight Marker XIII
Rely on DNA Molecular Weight Marker XIII for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 50 – 750 bp showing 15 fragments
of: 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750
and an additional band of 2642 bp. The 250 and 500 bp banding pattern is two
to three times brighter.
DNA Molecular
Weight Marker XIV (100 bp ladder)
Rely on DNA Molecular Weight Marker XIV for accurate molecular weight determination
on agarose gels of double-stranded DNA fragments generated by PCR or restriction
digests. It covers the size range from 100 – 1500 bp showing 15 fragments
of: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400,
1500 and an additional band of 2642 bp. The 500 and 1000 bp banding pattern
is two to three times brighter.
DNA Molecular
Weight Marker XV (Expand DNA Molecular Weight Marker)
Rely on DNA Molecular Weight Marker XV for accurate molecular weight
determination on agarose gels of double-stranded DNA fragments generated by
PCR or restriction digests. It covers the size range from 2.3 – 48.5 kbp
showing 24 fragments of:
2392, 3574, 7601, 8113, 9688, 10086, 11205, 11848, 12379, 13282, 14183, 15258,
15262, 16710, 18780, 19944, 20323, 22010, 24918, 26718, 29027, 32745, 38412,
48502 (undigested lambda DNA). The DNA fragments 15262 (15258), and the DNA
fragments 19944 (20323) appear as one single band on a 0.4% agarose gel. The
DNA fragments 9688, 13282, 22010 bp appear brighter on a 0.4% agarose gel.
DNA Molecular
Weight Marker XVI (250 bp ladder)
Rely on DNA Molecular Weight Marker XVI for accurate molecular weight
determination on agarose gels of double-stranded DNA fragments generated by
PCR or restriction digests. It covers the size range from 0.25 – 3.0 kbp
showing 12 fragments of: 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250,
2500, 2750, 3000 bp. This marker shows uniform spacing for easier reading, while
bands 1000 and 2000 appear 3x brighter for easy orientation.
DNA Molecular
Weight Marker XVII (500 bp ladder)
Rely on DNA Molecular Weight Marker XVII for accurate molecular weight
determination on agarose gels of double-stranded DNA fragments generated by
PCR or restriction digests. It covers the size range from 0.5 – 5.0 kbp
showing 10 fragments of: 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500,
5000 bp. This marker shows uniform spacing for easier reading, while bands 2000
and 4000 are 3x brighter for easy orientation.
RNA Molecular
Weight Marker I, DIG-labeled
Rely on RNA Molecular Weight Marker I for accurate molecular weight determination
on agarose gels of RNA fragments. It covers the size range from 0.3 - 6.95 kbp
showing 9 fragments of: 310, 438, 575, 1049, 1517, 1821, 2661, 4742, 6948 bp.
The DIG-labeled version can be used as size standards in northern blot analysis
when the DIG System for Nucleic Acid Labeling and Detection is used.
RNA Molecular
Weight Marker II, DIG-labeled
Rely on RNA Molecular Weight Marker II for accurate molecular weight determination
on agarose gels of RNA fragments. It covers the size range from 1.5 - 6.9 kbp
showing 5 fragments of: 1517, 1821, 2661, 4742, 6948 bp. The DIG-labeled version
can be used as size standards in northern blot analysis when the DIG System
for Nucleic Acid Labeling and Detection is used.
RNA Molecular
Weight Marker III, DIG-labeled
Rely on RNA Molecular Weight Marker III for accurate molecular weight determination
on agarose gels of RNA fragments. It covers the size range from 0.3 - 1.5 kbp
showing 5 fragments of: 310, 438, 575, 1049, 1517 bp. The DIG-labeled version
can be used as size standards in northern blot analysis when the DIG System
for Nucleic Acid Labeling and Detection is used.