PCR product labeling kits
Biotin-16-dUTP
Use Biotin-16-dUTP for enzymatic DNA labeling. The labeled DNA can subsequently
be detected by ELISA using a streptavidin-AP conjugate for nucleic acid detection.
Biotin-16-dUTP can replace dTTP in nick translation as well as in random-primed
DNA labeling. The nucleotide is further used as a substrate for E. coli DNA
polymerase I, T4 DNA polymerase, and terminal transferase.
Biotin-16-UTP
Use Biotin-16-UTP to replace UTP in the in vitro transcription reaction
for RNA labeling. The labeled RNA can subsequently be detected by ELISA using
a streptavidin AP conjugate for nucleic acid detection. Biotin-16-UTP is a substrate
for SP6, T3, and T7 RNA Polymerases.
Biotin-16-ddUTP
Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides
with terminal transferase. It is also a substrate for E. coli DNA Polymerase
I (holoenzyme and Klenow fragment), T4 and T7 DNA Polymerases, Taq DNA Polymerase,
and reverse transcriptase (e.g., from Transcriptor and M-MuLV). Labeled
oligonucleotides can be subsequently detected by ELISA using the streptavidin-AP
conjugate for nucleic acid detection.
Biotin RNA Labeling
Mix
Use the Biotin RNA Labeling Mix for labeling probes which are applied for Southern,
northern, dot blots, colony and plaque lifts, or in situ hybridization.
This ready-to-use mix is a 10 x conc. premixed solution of ATP, CTP, and GTP,
each at a concentration of 10 mM, UTP at a concentration of 6.5 mM, and biotin-16-UTP
at a concentration of 3.5 mM in water, pH 7.5.
Digoxigenin-11-UTP
Use Digoxigenin-11-UTP to replace UTP in the in vitro transcription
reaction for RNA labeling. The labeled RNA can subsequently be detected with
the Anti-DIG-AP, Fab fragments, the DIG Nucleic Acid Detection Kit, or with
the DIG Luminescent Detection Kit for nucleic acids. Digoxigenin-11-UTP is used
as a substrate for SP6, T3, and T7 RNA Polymerase. It is provided as a 10 mM
and a 3.5 mM solution of the Li-salt.
Digoxigenin-11-dUTP,
alkali-labile
Use DIG-11-dUTP for enzymatic DNA labeling. The labeled DNA can subsequently
be detected with the DIG Nucleic Acid Detection Kit, or with the DIG Luminescent
Detection Kit for Nucleic Acids. The use of this alkali-labile formulation is
especially recommended when stripping and reprobing of Southern blots is planned.
DIG-11-dUTP can replace dTTP in nick translation, random-primed DNA labeling,
and in the polymerase chain reaction (PCR). The nucleotide is also used as a
substrate for the E. coli DNA Polymerase I (holoenzyme and Klenow fragment),
T4 and Taq DNA Polymerases, reverse transcriptase (e.g., from Transcriptor
and M-MuLV), and the terminal transferase (e.g., DNA 3'-end labeling).
Digoxigenin-11-dUTP,
alkali-stable
Use DIG-11-dUTP for enzymatic DNA labeling. The labeled
DNA can subsequently be detected with the DIG Nucleic Acid Detection Kit or
with the DIG Luminescent Detection Kit for Nucleic Acids. DIG-11-dUTP can replace
dTTP in nick translation, random-primed DNA labeling, and in the polymerase
chain reaction (PCR) for DNA labeling. The nucleotide is also used as a substrate
for the E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and Taq
DNA Polymerases, reverse transcriptase (e.g., from Transcriptor and M-MuLV),
and for the terminal transferase for DNA 3'-end labeling. Digoxigenin is bound
to the nucleotide part via an alkali-resistant ether linkage. When stripping
and reprobing of Southern blots is planned, the use of the alkali-labile DIG-11-dUTP
formulation is recommended.
Digoxigenin-11-ddUTP
Use Digoxigenin-11-ddUTP for 3'-end labeling of oligonucleotides
with terminal transferase. It is also a substrate for E. coli DNA Polymerase
I (holoenzyme and Klenow fragment), T4 and T7 DNA Polymerase, Taq DNA Polymerase,
and reverse transcriptase (e.g., from Transcriptor and M-MuLV). In
addition to common hybridization techniques, DIG labeled oligonucleotides are
especially useful for screening expression libraries for sequence specific DNA
binding proteins (e.g., transcription factors).
DIG Nucleic Acid Detection Kit
Digoxigenin Oligonucleotide 3’-End Labeling Kit, 2nd generation
Digoxigenin Oligonucleotide Tailing Kit, 2nd generation
Fluorescein-12-dUTP
Use Fluorescein-12-dUTP for enzymatic labeling of DNA. It can replace dTTP in
nick translation and in random-primed DNA labeling. The preparation is used
as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow
fragment), T4 and T7 DNA Polymerases, Taq DNA Polymerase, and reverse transcriptase
(e.g., from Transcriptor and M-MuLV). It is a substrate for DNA 3'-end
labeling using terminal transferase. Fluorescein-labeled probes are suitable
for use in in situ hybridization with direct fluorescence detection,
or detection by ELISA using anti-fluorescein-AP.
Fluorescein-12-UTP
Fluorescein-12-UTP is used as a substrate for SP6, T3,
and T7 RNA Polymerases. It can replace UTP in the in vitro transcription reaction
for RNA labeling. Labeled RNA can be subsequently detected by in situ hybridization
and direct fluorescence detection or detection by ELISA technique using anti-fluorescein-AP.
Fluorescein RNA
Labeling Mix
Use the Fluorescein RNA Labeling Mix for labeling probes which are applied in
in situ hybridization, Southern, Northern, dot blots, or colony and plaque lifts.
This ready-to-use mix is a 10 x conc. premixed solution of ATP, CTP, and GTP,
each at a concentration of 10 mM, UTP at a concentration of 6.5 mM and fluorescein-12-UTP
at a concentration of 3.5 mM in water, pH 7.5.
Oligo(dT)20
Probe, Biotin labeled
Use biotin-labeled oligo (dT)20 probe for hybridization with eucaryotic mRNA
and immobilisation on streptavidin-coated solid supports like magnetic particles
or PCR tubes.
PCR Fluorescein
Labeling Mix
The PCR Fluorescein Labeling Mix is used for enzymatic labeling of DNA with
fluorescein-dUTP by PCR, for the preparation of ISH probes and membrane hybridization
probes. Fluorescein-labeled probes are suitable for use in in situ hybridization
with direct fluorescence detection, or detection by ELISA using anti-fluorescein-AP.
Random Primed
DNA Labeling Kit
Use the Random Primed DNA Labeling Kit for the random-primed labeling of plasmid
and phage DNA with any type of [alpha32P]-dNTP. DNA can be routinely
labeled to a specific activity of 1.6 x 109 dpm/µg in 30 minutes
at 37°C.
Tetramethyl-Rhodamine-5-dUTP
Tetramethylrhodamine-5-dUTP is used for enzymatic DNA labeling. Rhodamine-labeled
probes show red fluorescence and are well suited for in situ hybridization using
direct fluorescence detection. Multiple fluorescence-labeling using fluorescein-12-dUTP
(yellow) is also possible.