Nucleotides
For your convenience, PCR-Grade Nucleotides are available as nucleotide sets (all 4 nucleotides in separate vials), as single nucleotides, as kits (dNTPs, buffer, and polymerase in separate vials), ready-to-use mixes (premixed solutions of all 4 nucleotides), and ready-to-use master mixes that contain polymerase, nucleotides, and buffer (premixed 2x solution).
Choose Roche Applied Science’s dNTPacks, convenient products that combine PCR-Grade Nucleotides, thermostable enzymes and enzyme blends, and all associated components such as buffers and PCR-enhancing additives in one convenient package. Each dNTPack contains the additive-free sodium salt nucleotides as a ready-to-use mix of all 4 nucleotides (10 mM of each dNTP). For a detailed description click here.
Ready-to-use mixes of all 4 nucleotides
PCR Nucleotide
Mix, PCR Grade
This ready-to-use nucleotide mix is a premixed solution of multiple PCR Grade
nucleotide sodium salts in water: dATP, dGTP, dCTP, and dTTP (each at a concentration
of 10 mM). This mix is optimized for use in all types of amplification reactions
and primer-extension reactions.
PCR Nucleotide
MixPLUS, PCR Grade
This ready-to-use nucleotide mix is a premixed solution of the sodium salts
of dATP, dGTP, and dCTP, each at a concentration of 10 mM, and dUTP at a concentration
of 30 mM in water. The incorporation of dUTP in place of dTTP allows the degradation
of contaminating PCR products from former reactions, using Uracil-DNA
Glycosylase (UNG) to prevent carryover contamination from previous amplifications.
Ready-to-use mixes containing nucleotides, buffer and polymerase
Pwo Master
The Pwo Master contains all the reagents required for
performing High Fidelity PCR (except template and primer) in a ready-to-use,
double-concentrated master mix. The Pwo Master results in approximately 18 times
the fidelity of Taq DNA Polymerase.
High Fidelity
PCR Master
Ready-to-use double-concentrated master mix of dNTPs,
polymerase blend with proofreading activity, and buffer.
PCR Master
Double-concentrated ready-to-use PCR master mix of dNTPs,
Taq DNA Polymerase, and buffer.
DOP PCR Master
Double-concentrated PCR master mix with Taq DNA polymerase,
dNTPs, buffer, oligonucleotides, and control DNA.
Kits containing nucleotides, buffer, and polymerase
PCR Core Kit
Contains Taq DNA Polymerase (250 u), dNTP stock solution
(dATP, dCTP, dGTP, dTTP, 10 mM each, 200 µl), buffers, and MgCl2
stock solution.
PCR Core KitPLUS
Contains Uracil DNA glycosylase, Taq DNA Polymerase, dNTP
mix (100 µl, dATP, dCTP, dGTP, 10 mM each, dUTP 30 mM, 100 µl),
buffers, and MgCl2 solution.
Deoxynucleoside Triphosphate Sets containing all 4 nucleotides in separate vials
Deoxynucleoside
Triphosphate Set, PCR Grade
The Deoxynucleoside Triphosphate Set contains 4 individual
vials of dATP, dCTP, dGTP, and dTTP, each as a 100mM solution of the Na salt.
PCR-Grade Nucleotides are optimized for use in all types of amplification reactions
and primer-extension reactions.
Deoxynucleoside
Triphosphate Set, Li-Salt
Use the Deoxynucleoside Triphosphate Set, Li-Salt for
DNA sequencing, labeling, and all types of amplification reactions or primer
extension reactions. It contains 4 individual vials of dATP, dCTP, dGTP, dTTP
each as a 100 mM solution of the Li-salt.
Single nucleotides
dATP, PCR Grade
dATP, PCR Grade contains 2´-Deoxyadenosine 5´-triphosphate
as a 100 mM solution of the Na-salt. PCR Grade Nucleotides are optimized for
use in all types of amplification reactions and primer-extension reactions.
dATP, Li-Salt
Use dATP, Li-Salt for DNA sequencing, labeling, and all
types of amplification reactions or primer extension reactions. It contains
2´-Deoxyadenosine-5´-triphosphate as a 100 mM solution of the Li-salt.
dCTP, PCR Grade
dCTP, PCR Grade contains 2'-Deoxycytidine 5'-triphosphate
as a 100 mM solution of the Na-salt. PCR Grade Nucleotides are optimized for
use in all types of amplification reactions and primer-extension reactions.
dCTP, Li-Salt
Use dCTP, Li-Salt for DNA sequencing, labeling, and all
types of amplification reactions or primer extension reactions. It contains
2´-Deoxycytidine-5´-triphosphate as a 100 mM solution of the Li-salt.
dGTP, PCR Grade
dGTP, PCR Grade contains 2'-Deoxyguanosine 5'-triphosphate
as a 100 mM solution of the Na-salt. PCR Grade Nucleotides are optimized for
use in all types of amplification reactions and primer-extension reactions.
dGTP, Li-Salt
Use dGTP, Li-Salt for DNA sequencing, labeling, and all
types of amplification reactions or primer extension reactions. It contains
2´- Deoxyguanosine-5´-triphosphate as a 100 mM solution of the Li-salt.
dTTP, PCR Grade
dTTP, PCR Grade contains 2'-Deoxythymidine 5'-triphosphate
as a 100 mM solution of the Na-salt. PCR Grade Nucleotides are optimized for
use in all types of amplification reactions and primer-extension reactions.
dTTP, Li-Salt
Use dTTP, Li-Salt for DNA sequencing, labeling, and all
types of amplification reactions or primer extension reactions. It contains
2´- Deoxythymidine-5´-triphosphate as a 100 mM solution of the Li-salt.
dUTP, PCR Grade
dUTP, PCR Grade contains 2'-Deoxyuridine 5'-Triphosphate
as a 100 mM solution of the Na-salt. PCR Grade Nucleotides are optimized for
use in all types of amplification reactions and primer-extension reactions.
dUTP, Li-Salt
Use dUTP, Li-Salt for DNA sequencing, labeling, and all
types of amplification reactions or primer extension reactions. It contains
2´-Deoxyuridine-5´-triphosphate as a 100 mM solution of the Li-salt.
dITP, Li-Salt
Use dITP, Li-Salt for the preparation of poly(dI) x poly(dC)
and poly[d(I-C)] with DNA Polymerase and dCTP. It contains 2´ -Deoxyinosine-5´
-triphosphate as a 100 mM solution of the Li-salt.
Modified nucleotides
Biotin-11-CTP
(Biotin-11-cytidine-5'-triphosphate)
Use Biotin-11-CTP to replace CTP in the in vitro transcription reaction
for RNA labeling. The labeled RNA can be subsequently detected with a fluorescent
streptavidin conjugate or by ELISA using a streptavidin AP conjugate. Biotin-11-CTP
is a substrate for SP6, T3, and T7 RNA Polymerases.
Biotin-16-UTP
(Biotin-16-uridine-5'-triphosphate)
Use Biotin-16-UTP to replace UTP in the in vitro transcription reaction
for RNA labeling. The labeled RNA can subsequently be detected by ELISA using
a streptavidin AP conjugate for nucleic acid detection. Biotin-16-UTP is a substrate
for SP6, T3, and T7 RNA Polymerases.
Biotin-16-dUTP
(Biotin-16-2'-deoxy-uridine-5'-triphosphate)
Use Biotin-16-dUTP for enzymatic DNA labeling. The labeled DNA can subsequently
be detected by ELISA using a streptavidin-AP conjugate for nucleic acid detection.
Biotin-16-dUTP can replace dTTP in nick translation as well as in random-primed
DNA labeling. The nucleotide is further used as a substrate for E. coli
DNA polymerase I, T4 DNA polymerase, and terminal transferase.
Biotin-16-ddUTP
Biotin-16-ddUTP is preferentially used for 3'-end labeling
of oligonucleotides with terminal transferase. It is also a substrate for E.
coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA Polymerases,
Taq DNA Polymerase, and reverse transcriptase (e.g., from Transcriptor
and M-MuLV). Labeled oligonucleotides can be subsequently detected by ELISA
using the streptavidin-AP conjugate for nucleic acid detection.
7-Deaza-dGTP
Use 7-Deaza-dGTP instead of dGTP in primer extension reactions
(e.g., sequencing according to Sanger) and receive a better resolution
of GC-rich regions. 7-Deaza-dGTP contains 7-deaza-2'-deoxy-guanosine-5'-triphosphate
as a 10mM solution of the Li-salt.
Digoxigenin-11-UTP
Use Digoxigenin-11-UTP to replace UTP in the in vitro
transcription reaction for RNA labeling. The labeled RNA can subsequently be
detected with the Anti-DIG-AP, Fab fragments, the DIG Nucleic Acid Detection
Kit, or with the DIG Luminescent Detection Kit for nucleic acids. Digoxigenin-11-UTP
is used as a substrate for SP6, T3, and T7 RNA Polymerase. It is provided as
a 10 mM and a 3.5 mM solution of the Li-salt.
Digoxigenin-11-dUTP,
alkali-stable
Use DIG-11-dUTP for enzymatic DNA labeling. The labeled
DNA can subsequently be detected with the DIG Nucleic Acid Detection Kit or
with the DIG Luminescent Detection Kit for Nucleic Acids. DIG-11-dUTP can replace
dTTP in nick translation, random-primed DNA labeling, and in the polymerase
chain reaction (PCR) for DNA labeling. The nucleotide is also used as a substrate
for the E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and Taq
DNA Polymerases, reverse transcriptase (e.g., from Transcriptor and M-MuLV),
and for the terminal transferase for DNA 3'-end labeling. Digoxigenin is bound
to the nucleotide part via an alkali-resistant ether linkage. When stripping
and reprobing of Southern blots is planned, the use of the alkali-labile DIG-11-dUTP
formulation is recommended.
Digoxigenin-11-dUTP,
alkali-labile
Use DIG-11-dUTP for enzymatic DNA labeling. The labeled
DNA can subsequently be detected with the DIG Nucleic Acid Detection Kit, or
with the DIG Luminescent Detection Kit for Nucleic Acids. The use of this alkali-labile
formulation is especially recommended when stripping and reprobing of Southern
blots is planned. DIG-11-dUTP can replace dTTP in nick translation, random-primed
DNA labeling, and in the polymerase chain reaction (PCR). The nucleotide is
also used as a substrate for the E. coli DNA Polymerase I (holoenzyme
and Klenow fragment), T4 and Taq DNA Polymerases, reverse transcriptase (e.g.,
from Transcriptor and M-MuLV), and the terminal transferase (e.g., DNA 3'-end
labeling).
Digoxigenin-11-ddUTP
Use Digoxigenin-11-ddUTP for 3'-end labeling of oligonucleotides
with terminal transferase. It is also a substrate for E. coli DNA Polymerase
I (holoenzyme and Klenow fragment), T4 and T7 DNA Polymerase, Taq DNA Polymerase,
and reverse transcriptase (e.g., from Transcriptor and M-MuLV). In
addition to common hybridization techniques, DIG labeled oligonucleotides are
especially useful for screening expression libraries for sequence specific DNA
binding proteins (e.g., transcription factors).
Fluorescein-12-dUTP
Use Fluorescein-12-dUTP for enzymatic labeling of DNA.
It can replace dTTP in nick translation and in random-primed DNA labeling. The
preparation is used as a substrate for E. coli DNA Polymerase I (holoenzyme
and Klenow fragment), T4 and T7 DNA Polymerases, Taq DNA Polymerase, and reverse
transcriptase (e.g., from Transcriptor and M-MuLV). It is a substrate
for DNA 3'-end labeling using terminal transferase. Fluorescein-labeled probes
are suitable for use in in situ hybridization with direct fluorescence
detection, or detection by ELISA using anti-fluorescein-AP.
Fluorescein-12-UTP
Fluorescein-12-UTP is used as a substrate for SP6, T3,
and T7 RNA Polymerases. It can replace UTP in the in vitro transcription reaction
for RNA labeling. Labeled RNA can be subsequently detected by in situ hybridization
and direct fluorescence detection or detection by ELISA technique using anti-fluorescein-AP.
5'-Methyl-2'-deoxycytidine-5'-triphosphate
Use 5-methyl-2'-deoxy-cytidine-5'-triphosphate for the
in vitro synthesis of hemi-methylated DNA.
Tetramethyl-rhodamine-5-dUTP
Tetramethylrhodamine-5-dUTP is used for enzymatic DNA
labeling. Rhodamine-labeled probes show red fluorescence and are well suited
for in situ hybridization using direct fluorescence detection. Multiple fluorescence-labeling
using fluorescein-12-dUTP (yellow) is also possible.
Dideoxynucleotides
Dideoxynucleoside
Triphosphate Set, Sequencing Grade
The Dideoxynucleoside Triphosphate Set contains 4 individual
vials of ddATP, ddCTP, ddGTP, and ddTTP, each as a 10 mM solution of the Na-salt
in water. Sequencing-Grade Dideoxynucleotides are optimized for use in sequencing
and cycle-sequencing reactions.
Dideoxynucleoside
Triphosphate Set, Li-Salt
Use the Li-salt solution of dideoxynucleoside triphosphates for DNA sequencing,
labeling, or primer extension reactions. The 2',3'-dideoxynucleoside triphosphates
inhibit the chain elongation of a given primer catalyzed by Klenow enzyme, a
feature for DNA sequencing according to Sanger. Dideoxynucleoside Triphosphate
Set, Li-Salt contains 4 individual vials of ddATP, ddCTP, ddGTP, ddTTP each
as a 10 mM solution of the Li-salt in water.
Ribonucleosides
Ribonucleoside
Triphosphate Set, Li-Salt
The Ribonucleoside Triphosphate Set is ideal for in vitro transcription reactions
and RNA polymerase-directed DNA sequencing. It contains 4 individual vials of
ATP, CTP, GTP, and UTP each as a 100 mM solution of the Li-salt.