Products for two step RT-PCR
Transcriptor High
Fidelity cDNA Synthesis Kit
Profit from the effective proofreading activity of Transcriptor High Fidelity cDNA Synthesis Kit
and obtain more accurate and sensitive two-step RT-PCR results on any real-time instrument or thermal cycler.
Take no risk by endangering your RT-PCR results by base exchanges or frameshifts which are further propagated
in subsequent PCR runs by DNA polymerases. In addition, get results faster. The kit includes
all reagents for two-step RT-PCR including two primers (anchored-oigo(dT) and random primer) and control reactions.
Transcriptor
First Strand cDNA Synthesis Kit
This new kit is designed for the robust and
sensitive reverse transcription of RNA from a variety of sources, in combination
with conventional thermal cyclers and real-time PCR Instruments.
Transcriptor
Reverse Transcriptase
Transcriptor Reverse Transcriptase is a fast new recombinant reverse transcriptase
expressed in E. coli. It includes a RNA-directed DNA polymerase, a DNA dependent
DNA polymerase, RNase H and unwinding activity. As template ss DNA and ss RNA
are accepted in the presence of a primer. Transcriptor Reverse Transcriptase
transcribes long RNAs and is recommended for RT-PCR because of its high sensitivity
in connection with high thermostability. Transcriptor Reverse Transcriptase
is also recommended for GC-rich templates and for labeling reactions during
cDNA synthesis.
5´/3´
RACE Kit, 2nd Generation
The 5'/3' RACE Kit has been developed to amplify DNA sequences from a messenger
RNA (mRNA) template between a defined internal site and unknown sequences of
either the 3’ or the 5’end of the mRNA. The kit contains all necessary
components for performing RACE including Reverse Transcriptase, dNTPs, buffers,
controls, primers, and the High Pure PCR Product Purification Kit.
AMV Reverse Transcriptase
Reverse Transcriptase AMV can be used for first- and second-strand cDNA synthesis
and dideoxy DNA sequencing. The enzyme can also be used for primer extension,
RNA sequencing, 3'-end labeling of DNA fragments, generation of single-stranded
probes for hybridization, and in genomic footprint experiments.
cDNA Synthesis
System
Use the cDNA Synthesis System for the synthesis of ds cDNA from total RNA or
mRNA. The ds cDNA can be used for many applications:
the construction of non-directional cDNA libraries, as a starting point for
substractive hybridization experiments to enrich differential expressed genes
and - as a major application - for the in vitro transcription of whole
cDNA populations to get labeled cRNA for hybridization on DNA microarrays.
Expand Reverse
Transcriptase
Expand Reverse Transcriptase is ideally suited for RT-PCR, where
amplification of cDNA fragments up to 13.5 kb from human dystrophin RNA is achieved
(using Expand Reverse Transcriptase and Expand Long Template PCR System).
First Strand cDNA Synthesis Kit
for RT-PCR (AMV)
Use the First Strand cDNA Synthesis Kit for two-step RT-PCR on thermal cycler or any real-time instrument.
The kit includes all reagents for two-step RT-PCR including two primers (oligo-dT and random primer) and
control reactions. The high reaction temperature of AMV compared to M-MuLV RT allows the reaction to be
performed at 42°C, giving higher specificity and better resolution of secondary structures compared to
a reaction performed at 37°C.
M-MuLV Reverse
Transcriptase
Reverse Transcriptase M-MuLV is used for in
vitro synthesis of cDNA transcripts of specific RNA sequences, the preparation
of cDNA libraries, or synthesis of first-strand cDNA for use in subsequent amplification
reactions (RT-PCR).
p(dN)6
Primer for cDNA Synthesis
Use the Hexanucleotide Mix, 10 x conc. for cDNA synthesis. The mix consists
of a mixture of random hexanucleotides dissolved in reaction buffer. The oligonucleotides
are HPLC purified, desalted, and phosphorylated at the 5’-end.
p(dT)10
Primer for cDNA Synthesis
Primer for cDNA synthesis, p(dT)10 consists of a decamer oligonucleotide
that shows tymidine (T) at each individual position. The oligonucleotides are
HPLC purified, desalted and supplied as a lyophilisate of the Li-salt. The p(dT)10
primers are phosphorylated at the 5’-end.
p(dT)15
Primer for cDNA Synthesis
Primer for cDNA synthesis. p(dT)15 consists of a 15-mer oligonucleotide that
shows tymidine (T) at each individual position. The oligonucleotides are HPLC
purified, desalted, and supplied as a lyophilisate of the Li-salt. The p(dT)15
primers are phosphorylated at the 5'-end.
Protector RNase
Inhibitor
Protector RNase Inhibitor is used to protect mRNA. Protector RNase Inhibitor
inhibits a wide spectrum of different RNases. It is useful in any application
where RNases could be a potential problem.
RNAse H
RNase H is a non-specific endoribonuclease which specifically cleaves RNA in
RNA:DNA hybrids. The RNA is cleaved by RNase H to release 5'-oligoribonucleotides.
Tth DNA Polymerase
This recombinant enzyme has both intrinsic transcriptase and thermostable DNA
Polymerase activity - a convenient solution for single tube RT-PCR. Tth
DNA Polymerase also tolerates temperatures up to 50°C – 70°C
for the RT reaction, and up to 95°C for the PCR, thereby overcoming problems
caused by RNA secondary structures. Carry-Over prevention – via the incorporation
of dUTP and subsequent treatment with UNG – is possible with Tth
DNA Polymerase.