Products for one step RT-PCR
Transcriptor One-Step RT-PCR Kit
Detect even low-copy number RNA - 1 fg of total RNA or 1 copy of viral RNA (in vitro transcript) - in your sample by choosing the Transcriptor One-Step RT-PCR Kit — for fast, accurate, and specific results with high yield on standard block cyclers. Achieve high specificity and reduce primer-dimers. Count on excellent performance with the kit's proprietary hot start buffer (patents pending).
C. therm
DNA Polymerase One Step RT-PCR System
C. therm. Polymerase easily overcomes structure and sensitivity problems
encountered during RT-PCR reactions. This magnesium-dependent polymerase exhibits
optimal activity between 60°C and 70°C (maximum of 72°C), dramatically
reducing RNA secondary structure and increasing transcription efficiency. C.
therm. Polymerase One Step RT-PCR System is composed of a unique enzyme
mix containing the Klenow fragment of DNA polymerase from Carboxydothermus
hydrogenoformans and Taq
DNA Polymerase. This powerful polymerase mixture is designed for highly
specific and sensitive RT-PCR from RNA (total RNA, poly(A)+ RNA, or viral RNA)
using a convenient “one-step” protocol.
Protector RNase
Inhibitor
Protector RNase Inhibitor is used to protect mRNA. Protector RNase Inhibitor
inhibits a wide spectrum of different RNases. It is useful in any application
where RNases could be a potential problem.
Titan One Tube
RT-PCR System
The Titan One Tube RT-PCR System is designed for the sensitive, quick, and reproducible
analysis of RNA with high fidelity. The one-step reaction system will reverse
transcribe RNA into cDNA (with enzyme AMV
Reverse Transcriptase), and amplify the cDNA with a thermo cycling reaction
(using an optimized enzyme blend from Expand
High Fidelity PCR System). The Titan One Tube RT-PCR System is also available
as part of a complete kit for one-step RT-PCR - the Titan
One Tube RT-PCR Kit - including controls, control primers, nucleotides,
RNase inhibitor, and Water,
PCR Grade.
Titan One Tube
RT-PCR Kit
The Titan One Tube RT-PCR Kit provides the enzyme blend of the Titan
One Tube RT-PCR System together with all other reagents needed to perform
one-step RT-PCR, including controls, control primers, nucleotides, RNase inhibitor,
and Water, PCR Grade.
It is designed for quick, sensitive, reliable analysis of RNA with high fidelity.
In a single tube, the system will reverse transcribe RNA into cDNA, then amplify
the cDNA with a thermo cycling reaction. The enzyme AMV
Reverse Transcriptase and the Expand
High Fidelity PCR System are part of the kit.
Tth DNA Polymerase
This recombinant enzyme has both intrinsic transcriptase and thermostable DNA
Polymerase activity - a convenient solution for single tube RT-PCR. Tth
DNA Polymerase also tolerates temperatures up to 50°C – 70°C
for the RT reaction, and up to 95°C for the PCR, thereby overcoming problems
caused by RNA secondary structures. Carry-Over prevention – via the incorporation
of dUTP and subsequent treatment with UNG – is possible with Tth
DNA Polymerase.