Mastermixes for real-time PCR
FastStart Universal Probe Master (Rox)
Ready-to-use hot start reaction mix for qPCR and qRT-PCR on all real-time PCR systems requiring ROX reference dye. FastStart Universal Probe Master (Rox) includes a novel reference dye, which enables its use without modification or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX.
FastStart TaqMan®
Probe Master (Rox)
The FastStart TaqMan® Probe Master (Rox) is a ready-to-use reagent
mix that simplifies the preparation of reactions for qPCR and two-step qRT-PCR.
In combination with a real-time PCR instrument, suitable PCR primers, and a
hydrolysis probe, FastStart TaqMan® Probe Master (Rox) allows
very sensitive detection and quantification of defined DNA sequences. This formulation
contains the ROX Reference Dye.
FastStart Universal SYBR Green Master (Rox)
Ready-to-use hot start reaction mix for qPCR and qRT-PCR on all real-time PCR systems requiring ROX reference dye. FastStart Universal SYBR Green Master (Rox) includes a novel reference dye, which enables its use without modifications or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX.
FastStart SYBR
Green Master
The FastStart SYBR Green Master is a ready-to-use reagent mix that simplifies
the preparation of reactions for qPCR and two-step qRT-PCR using the intercalating
dye SYBR Green I for DNA detection and analysis. In combination with a real-time
PCR instrument and suitable PCR primers, FastStart SYBR Green Master allows
very sensitive detection and quantification of defined DNA sequences.
ROX Reference
Dye
The Reference Dye is a stock solution of ROX Reference Dye at a 1 mM concentration
in water. It can be used as an additive for running quantitative, real-time
DNA-detection assays, including qPCR and two-step qRT-PCR. The use of Reference
Dye allows compensation for variations in fluorescence between wells. The Reference
Dye is not required when using the LightCycler® Systems because
the LightCycler® Instruments are designed and constructed to
minimize well-to-well variations.
Mastermixes for real-time PCR on the LightCycler® Instruments
The LightCycler® master mixes have been adapted to the special rapid cycling environment of the LightCycler® Instrument and to the different probe chemistries supported by the system. For more information about LightCycler® reagents please consult the LightCycler® special interest site.