PCR Enzyme dNTPacks
Choose Roche Applied Science’s dNTPacks, convenient products that combine PCR-Grade Nucleotides, thermostable enzymes and enzyme blends, and all associated components such as buffers and PCR-enhancing additives in one convenient package. Each dNTPack contains the additive-free sodium salt nucleotides as a ready-to-use mix of all 4 nucleotides (10 mM of each dNTP). Click the links below for detailed information about Roche's PCR Enzyme dNTPacks:
Expand High Fidelity
PCR System, dNTPack
The Expand High Fidelity PCR System is an enzyme blend containing Taq
DNA Polymerase and a polymerase with proofreading activity. Expand High
Fidelity PCR System delivers superior results with two-fold higher yield and
three-fold greater fidelity compared to Taq
DNA Polymerase.
Expand High FidelityPLUS
PCR System, dNTPack
Expand High FidelityPLUS PCR System is a next-generation blend consisting
of Taq DNA Polymerase
and a novel proofreading protein, isolated and characterized by Roche Applied
Science. This protein mediates proofreading activity but has no polymerase activity
itself.
Expand Long Range,
dNTPack
Upgrade from the Expand Long Template PCR System and choose the new Expand Long
Range dNTPack for consistent amplification of long PCR products. Generate PCR
products of 5 to 25 kb from genomic DNA with this next-generation system from
the company that pioneered long-template PCR.
Expand 20kbPLUS
PCR System, dNTPack
The Expand 20 kbPLUS PCR System is a unique enzyme mix containing
Taq DNA Polymerase
and, a thermostable polymerase with proofreading activity. Select Expand 20
kbPLUS PCR System when amplifying genomic DNA fragments up to 35
kb.
FastStart High
Fidelity PCR System, dNTPack
The FastStart High Fidelity PCR System –
A new enzyme blend for hot start PCR - Combines all the benefits of FastStart
Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify
fragments up to 5 kb. It is the optimal product for multiplex
PCR.
FastStart Taq
DNA Polymerase, dNTPack
FastStart Taq DNA Polymerase is a thermostable, chemically modified form of
recombinant Taq DNA Polymerase.
This enzyme delivers superior results thanks to its unique enzyme design and
optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures
below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C.
GC-RICH PCR
System, dNTPack
The GC-RICH PCR System, a blend of Taq
DNA Polymerase and a proofreading polymerase, powers through templates that
are difficult or impossible to amplify with other polymerases and other blends
of polymerases. The enhanced processivity of the blend and the unique GC-RICH
Resolution Solution combine to deliver superior performance – especially
from problematic templates.
Pwo SuperYield
DNA Polymerase, dNTPack *
Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA
up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo
DNA Polymerase with a newly optimized buffer system. This new buffer system
enhances the enzymatic properties of the polymerase, resulting in higher yields
of the amplification reaction with 18-fold increased fidelity compared to Taq
DNA Polymerase.
Taq DNA Polymerase
(1U/µl), dNTPack
Taq DNA Polymerase is the recombinant form of the
enzyme from the thermophilic eubacterium Thermus
aquaticus BM, expressed in E. coli. The enzyme is highly purified
and is free of nonspecific endo- and exonucleases. Taq DNA Polymerase consists
of a single polypeptide chain with a molecular weight of approximately 95 kD.
It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease
activity.
Taq DNA Polymerase,
dNTPack
Taq DNA Polymerase is the recombinant form of the
enzyme from the thermophilic eubacterium Thermus
aquaticus BM, expressed in E. coli. The enzyme is highly purified
and is free of nonspecific endo- and exonucleases. Taq DNA Polymerase consists
of a single polypeptide chain with a molecular weight of approximately 95 kD.
It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease
activity.
Enzymes for PCR
Expand High Fidelity
PCR System
The Expand High Fidelity PCR System is an enzyme blend containing Taq
DNA Polymerase and a polymerase with proofreading activity. Expand High
Fidelity PCR System delivers superior results with two-fold higher yield and
three-fold greater fidelity compared to Taq
DNA Polymerase.
Expand High FidelityPLUS
PCR System
Expand High FidelityPLUS PCR System is a next-generation blend consisting
of Taq DNA Polymerase
and a novel proofreading protein, isolated and characterized by Roche Applied
Science. This protein mediates proofreading activity but has no polymerase activity
itself.
Expand Long Template
PCR System
Choose Expand Long Template PCR System for long and accurate PCR reactions.
Use this optimized enzyme blend and specially developed 3-buffer set to generate
PCR products from 5 to 20 kb in length. Even if Taq
DNA Polymerase were able to amplify fragments of comparable lengths, Expand
Long Template PCR System would deliver twice the fidelity and much higher yields.
Expand 20 kbPLUS
PCR System
The Expand 20 kbPLUS PCR System is a unique enzyme mix containing
Taq DNA Polymerase
and, a thermostable polymerase with proofreading activity. Select Expand 20
kbPLUS PCR System when amplifying genomic DNA fragments up to 35
kb.
FastStart High
Fidelity PCR System
The FastStart High Fidelity PCR System –
A new enzyme blend for hot start PCR - Combines all the benefits of FastStart
Taq DNA Polymerase with 4-fold higher accuracy and the abilty to amplify
fragments up to 5 kb. It is the optimal product for multiplex
PCR.
FastStart Taq
DNA Polymerase
FastStart Taq DNA Polymerase is a thermostable, chemically modified form of
recombinant Taq DNA Polymerase.
This enzyme delivers superior results thanks to its unique enzyme design and
optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures
below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C.
GC RICH PCR
System
The GC-RICH PCR System, a blend of Taq
DNA Polymerase and a proofreading polymerase, powers through templates that
are difficult or impossible to amplify with other polymerases and other blends
of polymerases. The enhanced processivity of the blend and the unique GC-RICH
Resolution Solution combine to deliver superior performance – especially
from problematic templates.
Pwo SuperYield
DNA Polymerase*
Pwo SuperYield DNA Polymerase is ideal for high-fidelity amplification of DNA
up to 3 kb. Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo
DNA Polymerase with a newly optimized buffer system. This new buffer system
enhances the enzymatic properties of the polymerase, resulting in higher yields
of the amplification reaction with 18-fold increased fidelity compared to Taq
DNA Polymerase.
Taq DNA Polymerase,
1 U/μl
Taq DNA Polymerase is the recombinant form of the
enzyme from the thermophilic eubacterium Thermus
aquaticus BM, expressed in E. coli. The enzyme is highly purified
and is free of nonspecific endo- and exonucleases. Taq DNA Polymerase consists
of a single polypeptide chain with a molecular weight of approximately 95 kD.
It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease
activity.
Taq DNA Polymerase,
5 U/μl
Taq DNA Polymerase is the recombinant form of the
enzyme from the thermophilic eubacterium Thermus
aquaticus BM, expressed in E. coli. The enzyme is highly purified
and is free of nonspecific endo- and exonucleases. Taq DNA Polymerase consists
of a single polypeptide chain with a molecular weight of approximately 95 kD.
It is a highly processive 5'-3' DNA polymerase that lacks 3'-5' exonuclease
activity.
Taq DNA Polymerase,
GMP Grade
Taq DNA Polymerase, GMP Grade gives outstanding lot-to-lot consistency. The
polymerase is manufactured under GMP conditions. It also fulfills the high quality
and documentation requirements of research and development laboratories in the
pharmaceutical and biotechnology industries. This top-performing enzyme also
provides convenient packaging options to meet your research needs.
*not available in the US