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DIG RNA Labeling Kit (SP6/T7)

For RNA labeling with digoxigenin-UTP by in vitro transcription with SP6 and T7 RNA polymerase.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Quantity
11175025910
1 kit for up to 2 x 10 labeling reactions $569.00
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Kit for the labeling of RNA with digoxigenin-UTP by in vitro transcription with SP6 and T7 polymerases. By this method, single-stranded RNA probes of known length are produced, which can be used in a variety of hybridization techniques.
 

Contents

  1. pSPT18 DNA, 0.25 mg/ml
  2. pSPT19 DNA, 0.25 mg/ml
  3. Control DNA 1, pSPT18-Neo, cleaved with Pvu II, 0.25 mg/ml
  4. Control DNA 2, pSPT19-Neo, cleaved with Pvu II, 0.25 mg/ml
  5. DIG-labeled Control RNA, DIG-labeled "antisense" neo RNA, 100 ng/µl
  6. Unlabeled Control RNA, neo poly (A) "sense" RNA, 200 µg/ml
  7. NTP Labeling Mixture, 10x concentrated
  8. Transcription Buffer, 10x concentrated
  9. DNase I, RNase-free, 10 U/µl
  10. Protector RNase Inhibitor, 20 U/µl
  11. SP6 RNA Polymerase, 20 U/µl
  12. T7 RNA Polymerase, 20 U/µl

 

Need Help?

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+1-800-428-2883


Technical Service:
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Roche Diagnostics Corporation
Roche Applied Science
9115 Hague Road
P.O. Box 50414
Indianapolis, IN 46250-0414 USA

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