LightCycler® 480 High Resolution Melting Dye
Fluorescent dye for detection of double-stranded DNA in real-time PCR and high resolution melting analysis.
For life science research only. Not for use in diagnostic procedures.
LightCycler® 480 ResoLight Dye is a generic dye that binds to double-stranded DNA. It can be used at saturating concentrations to achieve highly homogeneous staining of amplicons and measurement of DNA formation in real-time PCR and DNA double-strand dissociation in melting analysis.
The LightCycler® 480 ResoLight Dye shows an enhanced sharpness of fluorescent signals with an excitation and emission wavelength similar to fluorescein. This dye can be detected with most of the available real-time PCR instruments, without inhibiting the PCR enzyme.
The LightCycler® 480 ResoLight Dye is a generic dye that can be used at high concentrations to achieve highly homogeneous staining of amplicons without PCR inhibition. When used in melting curve analysis, it leads to enhanced sharpness of fluorescent signals compared to classical dyes, thus enabling evaluation of the melting behavior of amplicons at high resolution.