LightCycler® 480 Gene Scanning Software
For life science research only. Not for use in diagnostic procedures.
The LightCycler® 480 System offers high resolution melting-based gene scanning as an integrated solution on a plate-based Real-Time PCR Instrument. The hardware, software, and High Resolution Melting Master (containing a proprietary DNA-saturating dye) are optimally designed to work together to support this powerful method. The entire gene-scanning experiment can be done on the same instrument for throughputs of up to 384 samples; post-PCR analysis does not require a separate device.
The LightCycler® 480 Gene Scanning Software, an accessory module to the LightCycler® 480 System, is based upon a proprietary algorithm for melting curve grouping according to shape. Grouping can be performed in an automated fashion or by including standard samples with known sequences - if available - as a reference. Before the grouping is performed, the melting curve raw data are processed in three steps: (i) normalization, (ii) temperature-shifting, and (iii) difference-plotting. The easy-to-use LightCycler® 480 Gene Scanning Software:
- Integrates seamlessly into the LightCycler® 480 Software package.
- Supports the use of melting standards for known genotypes to make analysis more reliable.
- Allows easy access to other LightCycler® 480 analysis functions (e.g., Tm analysis to check PCR specificity, Cq calling to check PCR efficiency).
- LightCycler® 480 Gene Scanning Software CD (1.5 SW update)
- LightCycler® 480 Gene Scanning Software Manual
The LightCycler® 480 Gene Scanning Software scans PCR fragments for the presence of genetic variations (e.g., SNPs, mutations, and methylation) by analyzing melting curve data obtained with the LightCycler® 480 High Resolution Melting Master.
Learn more about High Resolution Melting and Gene Scanning with the LightCycler® 480 System.
Gene scanning on the LightCycler® 480 System is based on the analysis of melting curve data obtained at high resolution in the presence of ResoLight dye, a proprietary, DNA-saturating dye that requires much lower concentrations than other dyes. With this method, unknown sequence variations (e.g., SNPs in diploid samples) become apparent because they have significantly different melting curve shapes compared to those derived from wild-type samples. In many cases, the method is also capable of differentiating homozygote wild types from mutants. It can also be used to detect other types of variations (e.g., sequence changes in methylated, bisulphite-treated DNA).