LightCycler® Fluorescein CPG
3' Dye when labeling HybProbe probes for single and multicolor detection on all LightCycler® 480 and LightCycler® Carousel-Based System Instruments.
For life science research only. Not for use in diagnostic procedures.
Fluorescein long chain alkylamino Controlled Pore Glass (lcaa CPG, 500 Å) for labeling the 3' terminus of oligonucleotides.
LightCycler® Fluorescein CPG labels the 3' end of an oligonucleotide that is combined with a 5' LightCycler® Red-labeled oligonucleotide when using HybProbe probes for detection. This support can be used in the same manner as standard nucleoside supports since it contains a dimethoxytrityl group.
The LightCycler® Fluorescein CPG is derived from a pure dye isomer, and is attached via a linkage that is stable during oligonucleotide synthesis. Therefore, HPLC purification is simple and results in a well-defined product. HybProbe probes synthesized using the LightCycler® Fluorescein CPG are blocked at the 3' terminus, which prevents them from Taq DNA polymerase extension and exonuclease digestion. Thus, the resulting oligonucleotide is perfectly suited for mono- and dual-color experiments using the LightCycler® Carousel-Based System Instruments.
Spectral characteristics for an oligonucleotide labeled with LightCycler® Fluorescein:
Excitation maximum: 494 nm
Emission maximum: 519 nm (both measured in 2 mM Tris buffer, pH 8.3).