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RealTime ready Cell Lysis Buffer

Easy-to-use reagent for cell lysis, prior to real-time RT-PCR applications.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size
07248431001
5000 reactions

RealTime ready Cell Lysis Buffer is an easy-to-use reagent for the lysis of cultured cells prior to reverse transcription.

  • Increase convenience:
    Purify RNA using a simple one-step cell lysis procedure instead of cumbersome multi-step protocols.
    Easily store and reduce handling steps for large scale projects with this bulk pack size.
  • Speed time to result:
    Lyse samples in just 5 minutes at room temperature without further steps, unlike other lysis methods.
  • Benefit from broad applicability:
    Scale up from 3 to 30,000 cultured cells for real-time PCR applications in 96- to 1536-well formats, with or without automation.
  • Minimize RNA Loss
    Minimize RNA loss by utilizing the whole cell lysate as a starting material for RT-qPCR, when compared to column-based purification methods.
  • Minimize waste
    Limit the waste materials to be managed by the lab with a bulk format of 5000 reactions in one bottle

Contents


Ready-to-use Cell Lysis Buffer (200 ml)

The RealTime ready Cell Lysis Buffer is designed for the lysis of cultured cells. Cell lysates can then be directly used for RT-qPCR or cDNA synthesis followed by a qPCR step. The quality of RNA isolated is suitable for relative quantification of mRNA by qPCR. The RealTime ready Cell Lysis Buffer is designed for rapid, high-performance gene expression studies using cultured cells in RT-qPCR or qPCR applications. The buffer uses an advanced one-step protocol for lysing 3 to 30,000 cells within 5 minutes at room temperature. The resulting lysate can be used directly for downstream real-time PCR applications, eliminating the time-consuming RNA purification step and ensuring minimal loss of RNA. With this large pack size and easy-to-use protocol, the RealTime ready Cell Lysis Buffer can be easily adapted to high throughput experiments while minimizing waste materials. In addition, this buffer easily integrates into automated cell-based gene expression workflows.

The RealTime ready Cell Lysis Buffer is optimal when used with the following products for downstream cDNA synthesis and real-time PCR analysis: Protector RNase Inhibitor, Transcriptor cDNA Synthesis Kits, LightCycler® Master Mixes, Universal ProbeLibrary probes, RealTime ready assays, and LightCycler® Instruments.
 

Quality

The RealTime ready Cell Lysis Buffer is tested to meet specifications of RT-qPCR.

Product No: 07248431001