Set standards for high-performance real-time PCR

Amplify your success in real-time PCR applications with our LightCycler^® instruments. Choose the system that best suits your needs as an academic or corporate researcher, with highly flexible sample volumes and throughputs ranging from very low (8) to very high (1536) per run. All of our instruments provide outstanding heating and cooling rates as well as control of temperature homogeneity, enabling you to run reactions that are both accurate and fast.

LightCycler® 96 System	LightCycler® Nano System
The LightCycler® 96 System is an optimal mid-size, mid-throughput qPCR solution for academic research, providing an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software that is accessible to any user make it easy to obtain publication-ready results. Includes gradient function and options to work with PCR strips or plates. Find products > System Performance Find documents	The LightCycler® Nano System features a plug-and-play device with stylish design, making it the smallest and most silent Real-Time PCR System on the market. Intuitive software that runs on Windows and Mac Systems enables qPCR novices as well as experienced researchers to easily carry out gene expression analysis and genotyping studies in up to 4 x 8-well tube strips. Find products  > System Performance Find documents
LightCycler® 480 System	LightCycler® 1536 System
The LightCycler®480 System is a high-performance, flexible throughput PCR platform (exchangeable 96- or 384-multiwell plate format) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. First to match the performance of our capillary-based system also on plates, it is ideal for multiplexing and automated workflows. Find products  > System Performance Find documents	The LightCycler® 1536 Instrument is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Take advantage of the first plate-based real-time PCR instrument that provides 1,536 data points in a single PCR run in less than 50 minutes, supported by an innovative reagent concept, including a powerful concept for surveillance of reagent pipetting accuracy. Find products > System Performance Find documents
LightCycler® Carousel-Based System
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. Glass capillaries and air-mediated heating and cooling make this system still stand out in terms of unreached speed. Conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. Find products > System Performance Find documents

High-performance reagents for all PCR application needs

The reagents for all LightCycler^® System instruments are based on Roche Applied Science’s improved hot start PCR enzyme formulation, delivering exceptional sensitivity and specificity for real-time PCR assays. FastStart Essential Master Mixes are robust and perfect for the LightCycler^® Nano System, and are supplied in cost-effective bundles together with disposables. The convenient ready-to-use LightCycler^® 480 master mixes are specially designed to support each of the main real-time PCR applications (e.g., gene quantification, genotyping) for both standard and fast ramping PCR run protocols. Furthermore, these optimized master mixes offer extended room-temperature stability for maximum robustness on automated high-throughput workflows, and improved storage conditions for added convenience with daily use. In addition, reagents for the ultra-high throughput LightCycler^® 1536 System include an innovative surveillance of the PCR setup.

Get a head start on your gene expression analysis with RealTime ready function tested assays for all annotated human, mouse, and rat genes. Rely on function tested qPCR assays based on Universal ProbeLibrary (UPL) short hydrolysis probes, substituted with locked nucleic acids (LNA). Easily select assays for your targets of choice on the free, online platform, RealTime ready Configurator, and configure your own custom panel on LightCycler^® Multiwell Plates 96 and 384. The RealTime ready Configurator provides all assay details, bioinformatics background, and primer sequences for each assay after ordering.

For gene expression assays for organisms other than human, mouse, or rat, see the Universal ProbeLibrary.

For other techniques and tools in gene expression workflows, such as sample preparation, microarrays, and cDNA synthesis products, click here.

LightCycler^® Systems offer a wide range of methods to study genetic and epigenetic variation. For detection of known variants, two different methods of SNP analysis are available on the LightCycler^® Nano and LightCycler^® 480 Systems:

• The basic, most commonly used method is Endpoint Genotyping analysis using enzymatically cleaved hydrolysis probes.
• A more advanced method is Melting Curve Genotyping analysis using hybridizing HybProbe or SimpleProbe probes.

Gene Scanning is the discovery of new variants in target-gene derived amplicons. It can be easily carried out based on the analysis of the melting behavior of these amplicons at high resolution in the presence of a saturating, DNA-binding dye. When combined with DNA bisulfite modification protocols, such high resolution melting approaches can also be adapted to the study of methylation patterns.

For additional information, such as application notes, click here.

For products, click here.