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RealTime ready custom qPCR assays can be selected for any human, mouse, or rat targets of your choice. All assays are function tested and enable fast and efficient expression profiling of your genes of interest. RealTime ready assays are available as single assays for the selected target or pre-plated on LightCycler® 480 Multiwell Plates 96 or 384.
Learn more: RealTime ready RT-qPCR Assay and Panels - Simple Online Configuration and Ordering
Explore the great functionalities and wealth of information of the RealTime ready Configurator
Each RealTime ready assay is designed to detect most known isoforms of a human, mouse, or rat gene. You can search and select assays for your targets of choice using the RealTime ready Configurator, a free online portal.
RealTime ready Assays are designed using the proprietary ProbeFinder Software, which is based on optimized and verified algorithms. For a particular gene, the most representative transcript is chosen for assay design. This ensures comprehensive coverage of most known splice variants of a certain gene. Most RealTime ready Assays are intron-spanning, enabling reliable quantification unbiased by genomic DNA residues in the sample material.
For more information, download the White Paper “RealTime ready Assay Design”
For each RealTime ready Assay, the RealTime ready Configurator provides the following information:
Function tested RT-qPCR Assays for Any Annotated Human, Mouse, and Rat Gene
Rigorously tested on the LightCycler® 480 Instrument with species-specific universal biological samples, each assay is experimentally qualified to perform according to strict criteria derived from MIQE guidelines.
RealTime ready Custom Single Assays
Create your specific gene expression panel with RealTime ready assays of your choice on LightCycler® 480 Multiwell Plates 96 or 384, using the RealTime ready Configurator. A multitude of different layouts is available for configuration. Assays are pre-plated and dried down for a reaction volume of 20 µl on 96-well plates and 10 µl on 384-well plates. Just add cDNA and master mix.
Overview of all plate layouts and formats
RealTime ready Custom Panels on LightCycler® 480 Multiwell Plates, 96
Function tested human qPCR assays of your choice, pre-plated on LightCycler® 480 Multiwell Plates, 96. For panel configuration and ordering, go to the RealTime ready Configurator
> RealTime ready Custom Panels on LightCycler® 480 Multiwell Plates, 96
RealTime ready Custom Panels on LightCycler® 480 Multiwell Plates, 384
Function tested human qPCR assays of your choice, pre-plated on LightCycler® 480 Multiwell Plates, 384. For panel configuration and ordering, go to the RealTime ready Configurator.
> RealTime ready Custom Panels on LightCycler® 480 Multiwell Plates, 384
The RealTime ready Focus Panels are designed for expression profiling of genes from a variety of ready pathways, functional groups or gene superfamilies. The assays are conveniently pre-plated and dried-down on LightCycler® 480 Multiwell plates.
> RealTime ready Focus Panels
Use this free web-based tool to quickly search for assays, configure custom panels, and order online. Select assays from interactive pathway maps or gene lists ranging from “Apoptosis” to “WNT Signaling”. Discover a wealth of bioinformatics content and graphical displays of, for example, amplicon position or exon/intron boundaries
Browse the Configurator at https://configurator.realtimeready.roche.com/
Use the following description, tips, and suggestions to optimize your assay search on the Configurator.
Or, go directly to a specific product category:
For gene expression assays for organisms other than human, mouse, or rat, see the Universal ProbeLibrary.
Read recent articles for more information on applications and performance.
FAQs and interactive troubleshooting.
T. Hubbard et al.: The Ensembl genome database project; Nucleic Acids Research 2002 30(1):38-41.
P. Flicek et al.: Ensembl’s 10th year; Nucleic Acids Research 2010 38:D557-D562
D. Maglott et al.: Entrez Gene: gene-centered information at NCBI; Nucleic Acids Research 2011 Jan; 39:D52-7.
P.A. Fujita et al.: The UCSC Genome Browser database: update 2011; Nucleic Acids Research 2010 Oct 18.
D. Karolchik et al.: The UCSC Table Browser data retrieval tool; Nucleic Acids Research 2004 Jan 1; 32:D493-6.
K.D. Pruit et al.: NCBI Reference Sequences: current status, policy and new initiatives; Nucleic Acids Research 2009 Jan; 37:D32-6.
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