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Labeling and Detection

Roche Applied Science provides a wide variety of kits and individual reagents for the labeling and detection of nucleic acids by different methods. In addition to the DIG System, an alternative to hazardous radioactive isotopes, Roche Applied Science offers nucleotides and reagents for efficient and convenient labeling of nucleic acids with biotin, fluorescein, or rhodamine, and also provides appropriate detection reagents. Choose products for filter or in situ hybridization and select the most suitable labeling and detection method using your preferred hapten and application.

Check out our Labeling Guide to find the best labeling method for your application.

Read the following Application Note to learn how to apply the DIG System in in situ applications, and how to optimize probe sensitivity and specificity for greater hybridization consistency.
> A Method for High Quality Digoxigenin-Labeled RNA Probes for In Situ Hybridization

Learn how to handle chromogenic and chemiluminescent visualization assays.
> Detection of Alkaline Phosphatase

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Catalog Products are: For life science research only. Not for use in diagnostic procedures unless otherwise indicated.

Custom Biotech Products are: For further processing only unless otherwise indicated.

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Labeling Methods	DIG-Labeling Reagents		Other Labeling Reagents
PCR Labeling	Kits for labeling	PCR DIG Probe Synthesis Kit PCR ELISA, DIG Labeling
	Mixes for labeling without enzymes	PCR DIG Labeling Mix PCR DIG Labeling Mix^PLUS	PCR Fluorescein Labeling Mix
	Nucleotides for labeling	Digoxigenin-11-dUTP, alkali-stable Digoxigenin-11-dUTP, alkali-labile Deoxynucleoside Triphosphate Set	Biotin-16-dUTP Fluorescein-12-dUTP Tetramethyl-Rhodamine-5-dUTP
	Enzymes	Expand High Fidelity^PLUS PCR System	Expand High Fidelity^PLUS PCR System

Nick Translation	Kits for labeling		Nick Translation Kit
	Mixes for labeling without enzymes	DIG-Nick Translation Mix	Nick-Translation Mix Biotin-Nick-Translation Mix
	Nucleotides for labeling	Digoxigenin-11-dUTP, alkali-stable Digoxigenin-11-dUTP, alkali-labile Deoxynucleoside Triphosphate Set
	Enzymes	DNA Polymerase I DNase I recombinant, RNase-free	DNA Polymerase I DNase I recombinant, RNase-free

Random Priming	Kits for labeling and detection	DIG High Prime Labeling and Detection Starter Kit I DIG High Prime Labeling and Detection Starter Kit II DIG DNA Labeling and Detection Kit
	Kits for labeling	DIG DNA Labeling Kit	Random Primed DNA Labeling Kit
	Mixes for labeling without enzymes	DIG-High Prime DIG DNA Labeling Mix	High Prime Biotin-High Prime Fluorescein-High Prime
	Nucleotides for labeling	Digoxigenin-11-dUTP, alkali-stable Digoxigenin-11-dUTP, alkali-labile	Hexanucleotide Mix Biotin-16-dUTP Fluorescein-12-dUTP Tetramethyl-Rhodamine-5-dUTP
	Enzymes	Klenow Enzyme, labeling grade	Klenow Enzyme, labeling grade
	Additional products	Primer "random"	Primer "random"

In Vitro Transcription	Kits for labeling	DIG Northern Starter Kit DIG RNA Labeling Kit (SP6/T7)	SP6/T7 Transcription Kit
	Mixes for labeling without enzymes	DIG RNA Labeling Mix	Biotin RNA Labeling Mix Fluorescein RNA Labeling Mix
	Nucleotides for labeling	Digoxigenin-11-ddUTP	Biotin-16-dUTP Fluorescein-12-dUTP Tetramethyl-Rhodamine-5-dUTP
	Enzymes	SP6 RNA Polymerase T3 RNA Polymerase T7 RNA Polymerase	SP6 RNA Polymerase T3 RNA Polymerase T7 RNA Polymerase
	Additional products	Protector RNase Inhibitor	Protector RNase Inhibitor

Tailing	Kits for labeling	DIG Oligonucleotide 5'-End Labeling Set
	Nucleotides for labeling	Digoxigenin-11-dUTP, alkali-stable Digoxigenin-11-dUTP, alkali-labile	Biotin-16-dUTP Fluorescein-12-dUTP Tetramethyl-Rhodamine-5-dUTP
	Enzymes	Terminal Transferase	Terminal Transferase

3'-End Labeling	Kits for labeling	DIG Oligonucleotide 3'-End Labeling Kit, 2^nd generation DIG Gel Shift Kit, 2^nd generation
	Nucleotides for labeling	Digoxigenin-11-ddUTP	Biotin-16-ddUTP
	Enzymes	Terminal Transferase	Terminal Transferase

5'-End Labeling	Kits for labeling	DIG Oligonucleotide 5'-End Labeling Set
5'-End Labeling	Additional products	Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

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Labeling and Detection

The DIG System

Specifically Label and Detect Nucleic Acids

DNA Labeling Guide

RNA Labeling Guide

Oligonucleotide Labeling Guide

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