Product Catalog
Anti-Fluorescein-POD, Fab fragments from sheep | |
|
For the detection of fluorescein-labeled compounds. |
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| Catalog Number | Pack size | |
|---|---|---|
| 11426346910 | 150 U | Order and Price Information |
Application
For the detection of fluorescein-labeled compounds.
- Dot blot
- ELISA
- Immunohisto/cytochemistry
- In situ hybridization
- Southern blot
- Western blot
Product Description
Clone: polyclonal
Ig class: sheep IgG, Fab fragments
Specificity: The polyclonal antibodies react with free and bound fluorescein.
Working concentration:
Dot blot: 150 mU/ml
ELISA: 50 - 150 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 500 - 1,000 mU/ml
Ig class: sheep IgG, Fab fragments
Specificity: The polyclonal antibodies react with free and bound fluorescein.
Working concentration:
Dot blot: 150 mU/ml
ELISA: 50 - 150 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 500 - 1,000 mU/ml
Background Information
The reagent is an anti-fluorescein antibody, Fab
fragments from sheep, conjugated with horse-radishperoxidase (POD). After
immunization with fluorescein the sheep IgG was purified by ion exchange
chromatography and the specific IgG was isolated by immunosorption. The Fab
fragments obtained by papain digestion were purified by gel filtration,
conjugated with POD and stabilized in 60 mM Tris-Hepes-buffer, 0.4% bovine
immunoglobulin (w/v), 0.2% Germall II (w/v), pH 7.2.
Contents
Lyophilizate, stabilized
Additional Information
Product Literature
Instructions for Use and Material Safety Data Sheets
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