Product Catalog
Anti-Fluorescein-AP, Fab fragments from sheep | |
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For the detection of fluorescein-labeled compounds. |
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| Catalog Number | Pack size | |
|---|---|---|
| 11426338910 | 150 U 200 µl | Order and Price Information |
Application
For the detection of fluorescein-labeled compounds.
- Dot blot
- ELISA
- Immunohisto/cytochemistry
- In situ hybridization
- Southern blot
- Western blot
Product Description
Clone: polyclonal
Ig class: sheep IgG, Fab fragments
Specificity: The polyclonal antibodies react with free and bound fluorescein.
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Ig class: sheep IgG, Fab fragments
Specificity: The polyclonal antibodies react with free and bound fluorescein.
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Background Information
After immunization with fluorescein, the sheep IgG was purified by ion exchange chromatography and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated with AP, and stabilized in 50 mM triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 1% bovine serum albumin (w/v), pH 7.6.
Contents
Solution, stabilized
Additional Information
Product Literature
Instructions for Use and Material Safety Data Sheets
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