Product Catalog
Anti-Fluorescein from mouse IgG1 (clone B13-DE1) | |
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For the detection of fluorescein-labeled compounds. |
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| Catalog Number | Pack size | |
|---|---|---|
| 11426320001 | 100 µg | Order and Price Information |
Application
For the detection of fluorescein-labeled compounds.
- ELISA
- Immunohisto/cytochemistry
- In situ hybridization
- Western blot
Product Description
Clone: B13-DE1
IgG class: mouse IgG1
Specificity: The monoclonal antibody (clone B13-DE1) reacts with free and bound fluorescein.
Working concentration:
ELISA: 2 - 4 µg/ml
Immunohisto/cytochemistry: 0.5 - 2 µg/ml
In situ hybridization: 0.2 - 0.4 µg/ml
Western blot: 0.5 - 2 µg/ml
IgG class: mouse IgG1
Specificity: The monoclonal antibody (clone B13-DE1) reacts with free and bound fluorescein.
Working concentration:
ELISA: 2 - 4 µg/ml
Immunohisto/cytochemistry: 0.5 - 2 µg/ml
In situ hybridization: 0.2 - 0.4 µg/ml
Western blot: 0.5 - 2 µg/ml
Background Information
To obtain monoclonal antibodies, Balb/c mice were immunized with KLH-bound fluorescein and the spleen cells were subsequently fused with myeloma cells of the cell line NS-1. The antibodies were purified by gel filtration, diluted in 10 M potassium phosphate buffer, 75 mM NaCl, 2% raffinose (w/v), 0.01% 2-methylisothiazolone (MIT) (w/v), pH 7.4, and subsequently lyophilized.
Contents
Lyophilizate, stabilized
Additional Information
Product Literature
Instructions for Use and Material Safety Data Sheets
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