Product Catalog

Anti-Digoxigenin-POD (poly), Fab fragments

from sheep


Catalog NumberPack size
1163371600150 UOrder and Price Information
 


Application

For the detection of digoxigenin-labeled compounds.
  • Dot blot
  • ELISA
  • Southern blot
  • Western blot

Product Description

Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Working concentration:
Dot blot: 50 - 100 mU/ml
ELISA: 2 - 50 mU/ml
Western blot: 50 - 100 mU/ml
Southern blot: 50 - 100 mU/ml

Background Information

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Contents

Lyophilizate

Additional Information

US Material Safety Data Sheets

EC Material Safety Data Sheets

Package Inserts/Product Instructions


Special Interest Sites


Disclaimer

The labeling of nucleic acids with DIG is covered by EP patents 0 324 474 and 0 371 262 as well as the following US patents 5.344.757, 5.354.657 and 5.702.888 owned by Roche Diagnostics GmbH.

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