Product Catalog

Anti-Digoxigenin

clone 1.71.256, mouse IgG1κ


Catalog NumberPack size
11333062910100 µgOrder and Price Information
 


Application

For the detection of digoxigenin-labeled compounds.
  • ELISA
  • Immunohisto/cytochemistry
  • In situ hybridization
  • Western blot

Product Description

Specificity: The monoclonal antibody (clone 1.71.256) reacts with free and bound digoxigenin.
Working concentration:
ELISA: 2 - 4 µg/ml
Immunohisto/cytochemistry: 0.5 - 2 µg/ml
In situ hybridization: 0.2 - 0.4 µg/ml
Western blot: 0.5 - 2 µg/ml

Background Information

Preparation: Balb/c mice were immunized with edestin-bound digoxigenin; the spleen cells were subsequently fused with myeloma cells of the cell line P3X63Ag8.653. The antibodies were purified by gel filtration, diluted in 10 mM potassium phosphate, 75 mM NaCl, 5% raffinose (w/v), 0.1% Kathon CG (w/v), pH 7.8, then lyophilized.

Contents

Lyophilizate

Additional Information

US Material Safety Data Sheets

EC Material Safety Data Sheets

Package Inserts/Product Instructions


Special Interest Sites


Product Literature

Disclaimer

The labeling of nucleic acids with DIG is covered by EP patents 0 324 474 and 0 371 262 as well as the following US patents 5.344.757, 5.354.657 and 5.702.888 owned by Roche Diagnostics GmbH.

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