Product Catalog
ß-Galactosidase EIA grade | |
| Catalog Number | Pack size |
|---|
Application
Used for labeling in the field of enzyme immunoassay techniques. β-Galactosidase can be coupled to other proteins via its SH-groups. The reconstituted solution can be used directly for conjugation without prior dialysis.
Product Description
β-D-Galactoside galactohydrolase
EC 3.2.1.23
Specific activity: approx. 750 - 950 U/mg enzyme protein ≈ approximately 150 - 250 U/mg lyophilizate (37°C, 2-nitrophenyl-β-D-galactoside); approx. 250-400 U/mg enzyme protein ≈ approx. 60-100 U/mg lyophilizate (37°C, 4-nitrophenyl-β-D-galactoside)
EC 3.2.1.23
Specific activity: approx. 750 - 950 U/mg enzyme protein ≈ approximately 150 - 250 U/mg lyophilizate (37°C, 2-nitrophenyl-β-D-galactoside); approx. 250-400 U/mg enzyme protein ≈ approx. 60-100 U/mg lyophilizate (37°C, 4-nitrophenyl-β-D-galactoside)
Contents
Lyophilizate, stabilized with phosphate buffer and sucrose
Note: contains at least 12 free SH-groups/enzyme molecule; 5 mg approx. 20 mg lyophilizate, 25 mg approx. 100 mg lyophilizate
Note: contains at least 12 free SH-groups/enzyme molecule; 5 mg approx. 20 mg lyophilizate, 25 mg approx. 100 mg lyophilizate
Quality
Aggregated β-Galactosidase:≤3% (HPLC)
Free thiol groups:≥12 moles/mole β-Galactosidase (37°C, Ellman’s reagent)
Purity: single peak (HPLC)
Free thiol groups:≥12 moles/mole β-Galactosidase (37°C, Ellman’s reagent)
Purity: single peak (HPLC)
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