Product Catalog

The kit can efficiently isolate both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for:

• Ligation and transformation
• Enzymatic restriction
• Labelling by random primed or nick translation methods
• Sequencing 

• Quick and simple; extraction procedure requires approx. 45 min time and only a few hands-on steps.
• Works with different agaroses and buffer systems; low melting point agarose is not required. 
• Efficient; specific binding of DNA allows easy removal of impurities.
• Isolates large DNA fragments without shearing; silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments (up to 100 kb).
• Even isolates oligonucleotides (≥20 bp); narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix. 
• Free of fines and small particles; recovered product will not inhibit subsequent enzymatic reactions.
• Good yield; see "Product Description" for typical recoveries. 

Size range of recovered DNA: 20 bp - 100 kbp

Typical recovery: 20 bp, 55%; 40 bp, 68%, 120 bp, 76%; 200 bp, 80%; 8 - 9 kb, 75%; larger than 10 kb, <60%

1. Silica Matrix, for 100 standard reactions
2. Solubilization Buffer, 60 ml
3. Nucleic Acid Binding Buffer, 100 ml
4. Wash Buffer, 20 ml

Briefly, the extraction procedure involves the following:

1. DNA fragments are separated by electrophoresis until all interesting DNA fragments are resolved. A razor blade or scalpel is used to excise a minimal amount of agarose that contains each fragment.
2. The agarose is then solubilized and the solution is mixed with a silica suspension that contains a chaotropic salt. Under the buffer conditions used, DNA binds the silica matrix.
3. Since the binding process is specific for nucleic acids, the DNA remains bound while impurities are removed with simple washing steps.
4. The DNA is then eluted from the matrix in low salt buffer or water. Recovered DNA is ready-to-use for downstream applications.

Figure 1: Recovery of DNA fragments from agarose gels with the Agarose DNA Extraction Kit. DNA fragments (0.5 – 23 kb) from Roche Applied Science Molecular Weight Marker II (MWM II) were separated on a 1% agarose gel, then extracted from the gel with the Agarose Gel DNA Extraction Kit. Isolated fragments were displayed on a new agarose gel.
Lanes 1, 9: MWM II
Lane 2: 23 kb fragment
Lane 3: 9.5 kb fragment
Lane 4: 6.5 kb fragment
Lane 5: 4.3 kb fragment
Lane 6: 2.3 kb fragment
Lane 7: 2.0 kb fragment
Lane 8: 0.5 kb fragment
Result: The Agarose Gel DNA Extraction Kit isolated both large and small DNA fragments with good yields.

By combining agarose gel electophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 - 50 μl.

Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labelled to high specific activity. Restriction digests proceed without inhibition.

• Nick Translation Kit;
  For labeling DNA with radioactive or modified dNTPs.
• Agarose MS molecular screening agarose;
  
• Random Primed DNA Labeling Kit;
  For labeling DNA using random oligonucelotides as primers
• High Pure PCR Product Purification Kit;
  
• Agarose MP multipurpose agarose;
  
• PCR Core Kit;
  The PCR Core Kit contains all reagents, necessary to optimize conventional PCR conditions for fragments up to 3 kb.
• Rapid DNA Ligation Kit;
  For rapid and efficient DNA ligation.
• Biotin-Nick Translation Mix;
  
• Agarose LE low electroendosmosis;
  
• DIG-Nick Translation Mix;
  
• Nick Translation Mix;