Product Catalog

The incorporation of 7-deaza-2'-deoxyguanosine-5'-triphosphate (7-deaza-dGTP) into DNA can eliminate spurious GC-hydrogen bonding and relax secondary structures, an effect that may be useful in all types of dideoxy sequencing and polymerization techniques. For example:

• Sequencing: Use 7-deaza-dGTP) instead of dGTP in primer extension reactions (e.g., Sanger sequencing) to better resolve GC-rich regions on gels.
• PCR: Partial substitution of 7-deaza-dGTP for dGTP in PCRs can improve the product yield when the template is GC-rich (i.e., has extensive secondary structures). 

7-deaza-2'-deoxyguanosine-5'-triphosphate, Li⁺ salt

Formula: C₁₁H₁₇N₄O₁₃P₃

Molecular weight: 506.2

Form: clear, colorless solution in water

Concentration: 10 mM

pH: 7

Stability: stable for 12 months after the production date when stored at -15 to -25°C

Typical analysis: Preparation contains at least 95% 7-deaza-dGTP.

When PCR products contain 7-deaza dGTP, they do not stain well in ethidium bromide, presumably because stacking of adjacent bases in the products is diminished. 

PCR amplification efficiency is generally not affected by 7-deaza-dGTP.

• GC-RICH PCR System;
  Optimized system for amplification of difficult targets up to 5 kb by the polymerase chain reaction (PCR).
• T4 Gene 32 Protein;
  Is used as an additive to improve PCR results.
• Dideoxynucleoside Triphosphate Set Sequencing Grade, sodium salt;
  The Dideoxynucleoside Triphosphate Set, Sequencing Grade, is produced to the highest purity level for optimal use in molecular biology applications like Sanger sequencing or primer-extension reactions.
• pUC 18 DNA;
  
• High Pure PCR Product Purification Kit;
  
• Water, PCR Grade;
  Specially purified, double-distilled, deionized, and autoclaved water.
• FastStart Taq DNA Polymerase, dNTPack 5 U/µl;
  Thermally activated polymerase for hot start PCR amplification of genomic and cDNA targets up to 3 kb.
• GC-RICH PCR System, dNTPack;
  Optimized system for amplification of difficult targets up to 5 kb by the polymerase chain reaction (PCR), with ready-to-use PCR Nucleotide Mix.
• Taq DNA Polymerase, GMP Grade 5 U/µl;
  Achieve consistent results with our validated Taq DNA Polymerase, GMP Grade. Taq DNA Polymerase, GMP Grade, belongs to the family of high-performance amplification enzymes supplied by Roche Applied Science and is manufactured using validated production, quality control, and filling procedures.
• Klenow Enzyme Labeling grade, from Escherichia coli lysogenic NM 964;
  
• Tth DNA Polymerase;
  Thermostable DNA Polymerase with intrinsic reverse transcriptase activity for RT-PCR amplification of RNA to a length of at least 1 kb.
• FastStart Taq DNA Polymerase, 5 U/µl;
  Thermally activated polymerase for hot start PCR amplification of genomic and cDNA targets up to 3 kb.