Product Catalog

Sequence specificity
Acc I recognizes the sequence GT↓(A,C)(T,G)*A*C and generates fragments with 5´-cohesive termini.

Source
From flavobacterium species

Compatible ends
The Acc I site in all vectors containing a pUC/M13-derived polylinker generates a 5´CG overhang, which is compatible with fragments produced by the following restriction enzymes: Acy I, Cla I, Hin PI, Hpa II, Mae II, Msp I, Nar I, Sfu I, Taq I.

Isoschizomers
Acc I is an isoschizomer to Fbl I and Xmi I.

Methylation sensitivity
Acc I is inhibited by the presence of N6-methyladenine and 5-methylcytosine at the sites indicated (*) on the recognition sequence.

Activity in SuRE/Cut buffer System
Buffer printed in bold face type is the buffer recommended for optimal activity:

Relative activity in complete PCR mix
Taq DNA Polymerase PCR mix: <5% .

Incubation temperature
37°C

Unit definition
One unit is the enzyme activity that completely cleaves 1 μg λDNA in 1 h at 37°C in a total volume of 25 μl SuRE/Cut buffer A. Complete digestion of 1 μg    pBR322 DNA requires ca. 1 unit of Acc I.

Heat Inactivation
The enzyme can not be heat inactivated by incubating it for 15 min at 65°C.

Number of cleavage sites on different DNAs

1. Acc I
2. SuRE/Cut buffer A (10x)

Absence of nonspecific endonuclease activities
11 μg λDNA is incubated for 16 h in  50 μl   SuRE/Cut buffer A with excess Acc I. The number of enzyme units that may be included in the incubation mixture without changing the enzyme-specific pattern is stated under "Endo" (on the product label).

Ligation and recutting assay
Acc I fragments obtained by complete digestion of 1 μg pBR322 DNA are ligated for 16 h at 20°C with 0.5 U T4-DNA ligase in 10  μl buffer that contains 66 mM Tris-HCl, 5 mM MgCl₂, 5 mM dithioerythritol, 1 mM ATP, pH 7.5 (at 20°C). The percentages of product that can be ligated and subsequently recut with  Acc I (yielding the typical pattern of pBR322 × Acc I fragments) are stated under "Lig" and "Rec" (on the product label) .

Associated Products

• Alkaline Phosphatase, shrimp;
• Rapid DNA Ligation Kit;
• T4 RNA Ligase;
• Klenow Enzyme;
  labeling grade, DNA Polymerase I, large fragment
• DNA Molecular Weight Marker XIV (100 bp ladder);