Product Catalog

U-DNA cleavage can be used to increase the efficiency of site-directed mutagenesis. For this purpose, uracil is incorporated in vivo into the target DNA. After primer annealing, and enzymatic filling-in, and ligation, the selection for the mutated strand is performed by enzymatic degradation of the original non-mutated uracil, which contains the wild-type strand, using UNG. A further application is carryover prevention for PCR. During PCR, dUTP can be incorporated into the PCR product. Before a subsequent amplification reaction, contaminating PCR products are degraded using uracil-DNA glycosylase. The enzyme is inactivated at the beginning of the amplification reaction by heating to 95°C. Simultaneous strand cleavage of the U-DNA is achieved during this heating step.

The enzyme is supplied in storage buffer, 1 U/µl.

Function test: Carryover prevention activity is assayed by adding approximately 10⁵ dU that contains templates prior to the amplification reaction. After UNG treatment, no amplification products could be detected. The enzyme does not contain any contaminating exo- or endonucleases.

• PCR Core Kit^PLUS;
  This kit offers you to combine individualized PCR conditions with the prevention of carryover contamination for the amplification of fragments up to 3 kb in basic PCRs.
• Expand High Fidelity^PLUS PCR System;
  For the robust amplification of fragments up to 5 kb from all types of DNA by the polymerase chain reaction (PCR).
• High Pure PCR Template Preparation Kit;
  Low to medium throughput genomic DNA isolation.
• High Pure PCR Product Purification Kit;
  
• Agarose Gel DNA Extraction Kit;
  For the elution of DNA fragments from agarose gels.
• dUTP PCR Grade, sodium salt;
  Roche highest purity nucleotides for ambitious applications like PCR.
• Expand High Fidelity^PLUS PCR System, dNTPack;
  For the robust amplification of fragments up to 5 kb from all types of DNA by the polymerase chain reaction (PCR). Includes PCR Nucleotide Mix.
• Taq DNA Polymerase, 5 U/µl;
  Taq DNA Polymerase is a standard enzyme for the amplification of DNA fragments up to 3 kb by the polymerase chain reaction (PCR). It has neither proofreading nor hot start features.
• Taq DNA Polymerase, dNTPack 5 U/µl;
  Taq DNA Polymerase is a standard enzyme for the amplification of DNA fragments up to 3 kb by the polymerase chain reaction (PCR). It does not have proofreading or hot start features. Choose Roche Applied Science dNTPacks, convenient products that combine PCR grade nucleotides, thermostable enzymes and enzyme blends, and all associated components such as buffers and PCR-enhancing additives. Our PCR grade nucleotides are assayed for function in RT-PCR, ensuring optimal performance of all components. Each dNTPack contains the additive-free sodium salt nucleotides as a ready-to-use mix (10 mM of each dNTP).
• PCR Nucleotide Mix^PLUS;
  Highest purity nucleotides for ambitious applications like PCR.
• Uracil-DNA Glycosylase, heat-labile recombinant from marine bacterium BMTU 3346;
  UNG with an increased heat intolerance to make it the solution of choice for the use in PCR carryover prevention.