Product Catalog

Brief description: Alkaline phosphatase hydrolyzes 5´-monophosphate groups from both DNA and RNA. It can also hydrolyze 5´-diphosphate and 5´-triphosphate groups from RNA.

Source: calf intestine

Inactivation: Add 1/10 volume of EGTA to the reaction mix and incuabate at 65^oC for 10 min. To ensure complete inactivation of enzyme, extract the mix with phenol/chloroform/isoamyl alcohol to remove all protein. 

Preventing self-ligation of vectors during cloning
If a linearized vector lacks a 5´-phosphate, it cannot ligate to itself or form concatamers that contain multiple copies of the vector. Thus, the product of the ligation reaction is predominantly recombinant DNA (vector + DNA insert), rather than religated plasmid.

Solution, 1U/μl, in buffer, pH 7.6 (20^o C)

• DNA Molecular Weight Marker V;
  
• Taq DNA Polymerase, dNTPack 5 U/µl;
  Taq DNA Polymerase is a standard enzyme for the amplification of DNA fragments up to 3 kb by the polymerase chain reaction (PCR). It does not have proofreading or hot start features. Choose Roche Applied Science dNTPacks, convenient products that combine PCR grade nucleotides, thermostable enzymes and enzyme blends, and all associated components such as buffers and PCR-enhancing additives. Our PCR grade nucleotides are assayed for function in RT-PCR, ensuring optimal performance of all components. Each dNTPack contains the additive-free sodium salt nucleotides as a ready-to-use mix (10 mM of each dNTP).
• Taq DNA Polymerase, 5 U/µl;
  Taq DNA Polymerase is a standard enzyme for the amplification of DNA fragments up to 3 kb by the polymerase chain reaction (PCR). It has neither proofreading nor hot start features.
• Rapid DNA Ligation Kit;
  For rapid and efficient DNA ligation.