Product Catalog
Anti-Digoxigenin-AP, Fab fragments from sheep | |
| Catalog Number | Pack size | |
|---|---|---|
| 11093274910 | 200 µl 150 U | Order and Price Information |
Application
For the detection of digoxigenin-labeled compounds.
- Dot Blot
- ELISA
- Immunohisto/cytochemistry
- In situ hybridization
- Northern blot
- Southern blot
- Western blot
Product Description
Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Background Information
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Contents
Solution, stabilized
Additional Information
Special Interest Sites
Product Literature
Product Articles
- Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
- Rnase Protection Assay (RPA) Using DIG-Labeled RNA Probes
- The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
- A Simplified In Situ Hybridization Protocol for rare mRNAa
- Digoxigenin-based Method Using Chemiluminescence to detect DNA Sequence Ladders on Nylon Membranes
- The DIG System -- Nonradioactive and Highly Sensitive Detection of Nucleic Acids
- Northern Blot Analysis with Digoxigenin in PCR-labeled Probes in Research Samples fromMyocardial Biopsies
Instructions for Use and Material Safety Data Sheets
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