Product Catalog
Anti-Digoxigenin-POD, Fab fragments from sheep | |
| Catalog Number | Pack size | |
|---|---|---|
| 11207733910 | 150 U | Order and Price Information |
Application
For the detection of digoxigenin-labeled compounds.
- Dot blot
- ELISA
- Immunohisto/cytochemistry
- In situ hybridization
- Western blot
Product Description
Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Working concentration:
Dot blot: 150 mU/ml
ELISA: 50 - 150 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Working concentration:
Dot blot: 150 mU/ml
ELISA: 50 - 150 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml
Background Information
Preparation: After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Contents
Lyophilizate, stabilized
Additional Information
Special Interest Sites
Product Literature
Instructions for Use and Material Safety Data Sheets
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