Product Catalog

5-Bromo-2'-deoxy-uridine Labeling and Detection Kit I


Catalog NumberPack size
112967360011 kit, (for up to 100 tests)Order and Price Information
 


Show all Hide All

Application

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.

Benefits

  • Safe: No radioisotopes are used
  • Easy to perform: Follows a standard immunofluorescence protocol
  • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
  • Flexible: Allows double-labeling protocols

Product Description

Sample material: Cell culture: adherent cells, suspension cells, organ, or explant cultures. Tissue sections (after in vivo labeling with BrdU).
Specificity: Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2'-deoxy-uridine, and shows crossreactivity with 5-iodo-2'-deoxy-uridine (10%). Anti-BrdU shows no crossreactivity with 5'-fluoro-2'-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Background Information

Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2'-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.

Contents

  1. BrdU Labeling Reagent, 1,000x conc., sterile
  2. Washing Buffer concentrate, 10x
  3. Incubation Buffer
  4. Anti-BrdU, (contains nucleases for DNA denaturation)
  5. Anti-mouse-Ig-fluorescein

Principle

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU. The antibody to BrdU supplied with the kit contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments, which allow binding of the antibody to BrdU without destruction of the cellular morphology. A fluorescein-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. Subsequently, the sample is evaluated using an immunofluorescence microscope.

Figure 1: Principle of the BrdU Labeling and Detection Kit I (Fluorescein).

Additional Information

Disclaimer

Please go to the online technical support for regulatory and license disclaimers

Back To Top


This website contains information on products which is targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country. Please be aware that we do not take any responsibility for accessing such information which may not comply with any valid legal process, regulation, registration or usage in the country of your origin.