Product Catalog
Alkaline Phosphatase (AP) grade I, from calf intestine | |
| Catalog Number | Pack size | |
|---|---|---|
| 10108138001 | 1,500 U | Order and Price Information |
| 10108146001 | 7,500 U | Order and Price Information |
Application
Selective cleavage of terminal phosphate groups from oligonucleotides. Poly(U) is not cleaved.
Product Description
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
EC 3.1.3.1
Specific activity: approx. 2,000 U/mg at 37°C with 4-nitrophenyl phosphate as the substrate, and diethanolamine as the buffer (400 U/mg at 25°C with 4-nitrophenyl phosphate as the substrate, and glycine as the buffer)
EC 3.1.3.1
Specific activity: approx. 2,000 U/mg at 37°C with 4-nitrophenyl phosphate as the substrate, and diethanolamine as the buffer (400 U/mg at 25°C with 4-nitrophenyl phosphate as the substrate, and glycine as the buffer)
Contents
Suspension in 3.2 M ammonium sulfate solution, 1 mM MgCl2, 0.1 mM ZnCl2, pH approximately 7
Quality
Contaminants:<0.0004% PDE, <0.004% ADA and A-5-MP deaminase each
Additional Information
Instructions for Use and Material Safety Data Sheets
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