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LightCycler ® Systems for Real-Time PCR
Roche Applied Science 

LightCycler ® 2.0 Real-Time PCR System

The LightCycler ® 2.0 Real-Time PCR System is an innovative carousel/based thermal cycler platform with fluorescence detection system and high-quality reagents. It was the first system to introduce

  • hybridization probes
  • true melting curve analysis
  • automated absolute quantification
  • relative quantification with efficiency correction

PCR occurs in specially designed glass capillaries, which have an optimal surface-to-volume ratio to ensure rapid equilibration between the air and the reaction components. A typical amplification cycle requires only 30 to 60 seconds; an amplification reaction with 30 cycles is usually complete in about 30 minutes. To avoid contamination, the capillary is tightly closed; you do not need to open it at any time during analysis.

RAPID
Assay and analysis are complete in less than 45 minutes.

ACCURATE
Rely on ultra-precise hardware and optimized software to give you accurate and highly reproducible results.

SENSITIVE
Expect a greater signal-to-noise ratio and fewer unwanted PCR products, due to the rapid cycling of the LightCycler ® Instruments.

PROVEN
Trust the instruments that have already given your colleagues thousands of successful experiments.

 

LightCycler ® 480 Real-Time PCR System

The LightCycler ® 480 Real-Time PCR System is a high throughput gene quantification or genotyping real-time PCR platform with exchangable blocks for 96 and 384 samples in multiwell plates. It offers

  • enhanced throughput
  • compatibility with automation equipment
  • maximum flexibility regarding hardware and software

The LightCycler ® 480 Real-Time PCR System can complete a 348 well PCR run in 40 minutes.

The LightCycler ® 480 Real-Time PCR System features a compact and versatile benchtop instrument that includes interchangeable thermal block cycler units for 96- and 384-mulitwell plate formats. The system is operated by a separate desktop data station based on Windows XP.

SPEED & ACCURACY

  • Benefit from our novel thermal cycling and data capture technologies to achieve outstanding temperature homogeneity and assay reproducibility.
  • Increase the sensitivity and yield of your real-time PCR applications, with robust LightCycler ® 480 reagents and specially engineered consumables.
  • Analyze true raw data, breaking free from the need to use passive reference dyes or normalization plates to get accurate results.

VERSATILITY & CONVENIENCE

  • Choose either 96- or 384-well plate formats, exchanging thermal block cyclers in just a few minutes with no recalibration time.
  • Select from numerous assay formats and detection dyes, expanding the range of real-time PCR applications in your lab.
  • Discover the intuitive, user-friendly Roche software interface, including novel step-saving short cuts for high-throughput sample and data handling.

COMPATIBILITY & SECURITY

  • Take advantage of the LightCycler ® 480 Instrument's automation capabilities, integrating it as part of a LIMS in combination with robotic pipetting and plate loading.
  • Enjoy the extensive analysis capabilities of the modular LightCycler ® 480 Software, with high-end algorithms that ensure accuracy and reproducibility.
  • Know that your data are protected, using a system compatible with 21 CFR part 11 requirements.

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