Improved reproducibility. Faster publication. More trustworthy data. Get the most out of your gene expression research with the help of the MIQE guidelines for qPCR.
This website is designed to help you along every step of your workflow – from isolation to reverse transcription to analysis – while making it easier to collect the information necessary to make your research publication-ready and fully MIQE-compliant.
Proper preparation of your sample and isolation of RNA are the critical foundations for defining accurate results. Record key parameters in this phase such as handling, storage, extraction method and concentration to preserve RNA integrity and expression profiles.
Reverse transcribing mRNA into cDNA and, as an additional option for rare material, pre-amplifying this material are key steps for running your gene-specific assays. Follow the clear guidelines to ensure a contamination-free environment with consistent temperatures and conditions leading to high-quality reaction products.Download reverse transcription technical guide or application note
Here’s where careful preparation and planning finally pay off. Running the analysis phase of the experiment is critical to recording accurate and publication-ready results. The type of reagents, the cycling parameters of the PCR and even the software used to analyze the experiment should all be recorded with care to ensure a truly reproducible set of data.
Products will vary depending on workflow and usage. For specific options, contact your Roche sales representative.
With high-quality, MIQE-compliant results, you can get the most out of your research and ensure that your work is published faster. If you followed our online worksheet, you can print a publish-ready report or download our easy MIQE checklist to use in future research.
Be MIQE compliant from the start with one of our simplified Roche qPCR kits, based on your specific workflow.
Download our MIQE offer flyer and contact your Roche Sales Representative for your individual MIQE Starter Kit.