Nucleic Acid Isolation and Purification Manual, 3nd Edition

 

To select a chapter section, click on the corresponding page number.
The chapters below are in Acrobat PDF format .

Chapter 1
Introduction
Selection Guide 8
Product Overview 10 - 13
Overview of Nucleic Acid Purification and Isolation 14 - 15
Methods described in this manual 16


Chapter 2
High Pure Technology and Silicia Adsorption
Overview of Silicia Adsorption 18 - 20
High Pure PCR Template Preparation Kit 21 - 32
High Pure PCR Cleanup Micro Kit 33 - 39
High Pure PCR Product Purification Kit 40 - 46
High Pure Plasmid Isolation Kit 47 - 52
High Pure RNA Isolation Kit 53 - 61
High Pure RNA Tissue Kit 62 - 66
High Pure FFPE RNA Micro Kit 62 - 72
High Pure RNA Paraffin Kit 73 - 81
High Pure Viral RNA Kit 82 - 86
High Pure Viral Nucleic Acid Kit 87 - 91
High Pure 16 System Viral Nucleic Acid Kit 92 - 97
Troubleshooting Procedures for the High Pure Kits 98 - 107
Agarose Gel DNA Extraction Kit 108 - 112
High Pure 96 UF Cleanup Kit 113 - 118


Chapter 3
Ion Exchange Chromatography
Overview of Ion Exchange Chromatography 120 - 121
Genopure Plasmid Midi Kit 122 - 128
Genopure Plasmid Maxi Kit 129 - 135


Chapter 4
Solution-based Isolation
Overview of Solution-based Isolation 138 - 139
DNA Isolation Kit for Mammalian Blood 140 - 147
DNA Isolation Kit for Cells and Tissues 148 - 155
TriPure Isolation Reagent 156 - 163


Chapter 5
Affinity Purification
Overview of Affinity Purification 166 - 169
mRNA Capture Kit 170 - 174
mRNA Isolation Kit 175 - 182
RNA/DNA Stabilization Reagent for Blood/Bone Marrow175
mRNA Isolation Kit for Blood/Bone Marrow 184 - 191


Chapter 6
Gel Filtration
Overview of Gel Filtration 194 - 195
Quick Spin Columns 196 - 199
mini Quick Spin Columns 200 - 204


Chapter 7
Automated Nucleic Acid Isolation
MagNA Lyser Instrument 206 - 207
MagNA Pure LC Instrument 208
MagNA Pure Compact Instrument 209 - 210


Chapter 8
Premium Performance Products for PCR and RT-PCR
Introduction 212
PCR Product Selection Guide 213
RT-PCR Product Selection Guide 214
PCR Enzyme dNTPacks 214
Master Mixes 215
Real-Time PCR Instruments 216


Chapter 9
Appendix
Tips for Handling Nucleic Acids 218 - 219
Conversion Tables and Formulas 220 - 223
Buffers and Gels for Electrophoresis 224 - 226
Other Useful Information 226 - 227


Chapter 10
Ordering Guide
  230 - 237


Chapter 11
Index
  240 - 244


ras
Webmaster Roche Applied Science Home
© Roche Diagnostics Corporation See our legal Statement